XB-CLONE-3542751
Clone Name: IMAGE:7718341
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Gene:
hsd11b1l
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980531 [+]
gtaaacccagatttattacacaggaattgggctgaaatataattagtagttaaatgcattaaatagaaacagacagcaataacaaatgcagtgcaatgca
atataaaccacactagcattttagtcctgaaagccaacaatgttggggcccttaaacagcaatttacttccagaatgtttgaactttgtgttataacttc
atatgtatactgtcactttaatttaaagaatcatagcagctctaaatatagtaccaatgcattacactgtattttccatttgtagagcataaaagcagct
tttcctttacaaataaataatcttggctgtccaggaagggtacatctctccttttggaagtggcactcctattggaatcaaatgcacttgacaattgcac
tactggaatgccaaaaataagccagtgcattctggttgctgacagttgaggaatacagcttgagcattgtctctgtctagcatggctgctatgtttttgc
ttattgttctggatttttcctatacacacatattgtgtctatacttgaacactgttcatttattattaccaaattagtaggtgtgcatttgatgtgagtg
gttcccaggagttagtgctttgtttagggaattgtcttctcctcctacgagttataatggtacattctctggataaaccagtccctgtgttgtgggaacc
agtctctgaaaaagcaaagtggtcttacaaaccagggatagtacatctcccgctgtcggcctgctccagccgatacaacagcaagcgcagcatcacttgc
aggatacgc
3' EST: CX980530 [+]ggtagccccattgccagcagggggtatgtaaagtattaaccatagttacagagctgcataggctgtgtattctgtaatgctactgcattgtccattgctc
gggataaggggggacaggctggtatagcatgaatgtgcctcctcattaataagggtgcaaaaggggatcagcaatgatgatcccttaataaattcatgtt
tcatttactacttttcttacattttctgcaagtttaatccccagtcactgatccttcattgtgttttccaggttaattttctcagttacattcatttggc
cactgctgcacttccttacttgacacaatcaaaaggctcaattattgtgctttcatctttaacaggtaggatgagcagatactaagctgtcgatgttacc
agccctaaggctgatgccccatggtcagatccctgctgttgcgtgcaactaatcattccgagatgcctttctacgggcaacaataagagtcaccgatgga
ggggcctgcgcgttgctttggctttccgaactcgtgtgaagttgcctgcaggaggagactttgtacgacttcggggagccggggcgatgcgtgggccttt
ccacattgttgccggtggaaaggcatttcggagtggttggttattctgtgcggcatcagcctaaaacaattaaataataggttcaaagattgctccagtt
ctccctatcccactaaccatacttctttaccctaaccccaattaacccaccatcctatcacccaatcaactacagcctgacaganatgaaatatagccaa
gacaacaaaaggaaaatgtacagaacccatataaccctcactgtacattaaaaaaaaaaagatg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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