XB-CLONE-3542742
Clone Name: IMAGE:7718485
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Gene:
slc51al
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157205 [+]
TTTAGTCCCAGAGCCTCAGTGCCGGGCAAGGAGAGAGGGGGGCTGTTTTGTTCTGTGTCTCATAACCATTCATATACAGGAGCTGGGCTTCCGGGCACAA
GGAAAGCGCAAGAGAGGCGACAACCAGCAGGAAGACTCAACAAGAACAAGCCTGAAGCCCACTGACTAAATGGATCCTGAGCAAAATGATACCAAACCAC
CATTCAACCCCATCTGTGCCACCAGGCAGGCTCCTTACTCACATGAAATCCTAGAAAATCTTGATATAACTGGGATATTGCTCTTTGCTATTTTAACATT
TATGACTCTGGTATCACTGTTGGTGTTCTTGGAAGAAGCTTACTATATGTACAGAAAAATTCCAAACCCCAAAAATTCCATAATCATTTGGATAAATGCA
GGAGCCATGATGATTGCAACAACATCCTGCTTTGGAATGTGGATTCCACGTAGCACCATGTTTACTGACTTCACTGCTTCTGTGTTCCTTGCAGTGTTAA
TACACAAGTTTCAGCTCATGCTGGTAAATGAATGTGGGGGAAGAAGAGAATTTCTCTCAACATTTGGTGATACGAAGCTGAAGATTAGCACTGGCCCCTT
CTGCTGCTGTTGCCTGTGCCTGCCACACAAAGACATCAACAGAAAGACTTTATTCATCCTGAAGCTGGGTACATTTCAGTTTGCCTTCCTACGGCCGGTG
CTTATGTTTCTGGCTGTGGTTCTTTGGACAAATGGAACTTATATGATTGGTAATTCATCTGCAGAAAAAGCTACTATCTGGATTAATATTGGAGTTGGTA
TTACCACAATAACAGCCCTTTGGGCAGTGGGAATTATGTTTAATCTAGTAAAGGACAACTTAAAGGAGAAAAATATCATTGGCAAATTTGCAGTCTATCA
GTTTACAGTCATTTTGAGCCAACTGCAAACCTCAATCATTAATATTCTTGGTACAACCGGTGTCATCTCTTGTGTTCCTCCTCTCCCGGGGCCATCACGG
GCTTCCTACATGAACCAGCAGCTTCTCATTATGGAAATGTTCCTGGTCACAGTCATCTGCCGAGTGCTGTACCGGAGGAGATATGATGATAAAAATCTTT
TGGAAAACCAAGAGACCAATGATAATTTACGAAACAGCATGATGCATCTGAATGGCAAAGCTTTGGAAGATGGACCCCAAAGTGTTTAAAGGGCACCAAG
ACACATTATAATCTCAGTGTACCAACTGCAAGGTGTAGTGGCATCTCCAGAAATGCTACCCCTGGTCTGACAAAATGTGCAGTGAAATTGTGACCTTGTG
CCTCTGCCAATGCCCAACCAGAATGTACAGTGCAGCTGCTGGACATTCTAATGCTAAACTGCCGAGTAGTCACAGTAGTGAAGTGATAACTTCAAGCACA
GTGACCTCCAGGAGCAGTGGCAACCATACTAGCGCACTAAGGGGGTGGGCTGCCGCTCTACTTTGTACATATGTAGCTGGGTAACAACAACAAACACTGC
ATTGAACTTGGACATTTCACTTTTTTTGGGTGGGGGCTATAGAGTTATCATATATATCTTGTATGTATACAATTTGAGCATACTTGTTAAGTATTTGGAG
AAACCTTGTGAGTTCTTAATAAATGATAATGTATTTATAAAAAAAAA
5' EST: CX980537 [+]tttagtcccagagcctcagtgccgggcaaggagagaggggggctgttttgttctgtgtctcataaccattcatatacaggagctgggcttccgggcacaa
ggaaagcgcaagagaggcgacaaccagcaggaagactcaacaagaacaagcctgaagcccactgactaaatggatcctgagcaaaatgataccaaaccac
cattcaaccccatctgtgccaccaggcaggctccttactcacatgaaatcctagaaaatcttgatataactgggatattgctctttgctattttaacatt
tatgactctggtatcactgttggtgttcttggaagaagcttactatatgtacagaaaaattccaaaccccaaaaattccataatcatttggataaatgca
ggagccatgatgattgcaacaacatcctgctttggaatgtggattccacgtagcaccatgtttactgacttcactgcttctgtgttccttgcagtgttaa
tacacaagtttcagctcatgctggtaaatgaatgtgggggaagaagagaatttctctcaacatttggtgatacgaagctgaagattagcactggcccctt
ctgctgctgttgcctgtgcctgccacacaaagacatcaacagaaagactttattcatcctgaagctgggtacatttcagtttgccttcctacggccggtg
cttatgtttctggctgtggttctttggacaaatggaacttatatgattggtaattcatctgcagaaaaagctactatctggattaatattggagttggta
ttaccacataacagccctttgggcagtgngaattatgtttaatctangtaa
3' EST: CX980536 [+]tttttttttataatacattatcatttattaagaactcacaaggtttctccaaatacttaacaagtatgctcaaattgtatacatacaagatatatatgat
aactctatagcccccacccaaaaaaagtgaaatgtccaagttcaatgcagtgtttgttgttgttacccagctacatatgtacaaagtagagcggcagccc
acccccttagtgcgctagtatggttgccactgctcctggaggtcactgtgcttgaagttatcacttcactactgtgactactcggcagtttagcattaga
atgtccagcagctgcactgtacattctggttgggcattggcagaggcacaaggtcacaatttcactgcacattttgtcagaccaggggtagcatttctgg
agatgccactacaccttgcagttggtacactgagattataatgtgtcttggtgccctttaaacactttggggtccatcttccaaagctttgccattcaga
tgcatcatgctgtttcgtaaattatcattggtctcttggttttccaaaagatttttatcatcatatctcctccggtacagcactcggcagatgactgtga
ccaggaacatttccataatgagaagctgctggttcatgtaggaagcccgtgatggccccgggagaggaggaacacaagagatgacaccggttgtaccaag
aatattaatgattgaggtttgcagttggctcanaatgactgtaaactgatagactgcaaatttgccaatgatatttttctcctttaaagtgtcctttact
agattaaacataattcccactgcccaaagggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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