XB-CLONE-3542736
Clone Name: IMAGE:7718581
|
Gene:
atp6v0b
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX980541 [+]
cacaagggagaaagagcgaaacaaggttggacgcagagcaacaaagcatccttatccagctgtgccagaggcaagatggtcattagagtttatatcgcaa
cgtcttcgggctccacatctattaagaagaaacagcaagatgtcctagggtttttacatgccgttaaaattgaatttgaggagaaagacattgctgccaa
tgaggagaatcgaaagtggatgagagagaatattcctgagcattgccggccaacaagtggcaactcactgcctcctcagatcttcaacgacagccagtac
tgtggggattatgatgccttctttgaagcacgagagaataatgcagtctatgccttccttgggttgacagccccacctggttcaaaggaagctgaggctc
tggcaaagaagaatgcagaatcatcataactggaatatttcagaaccgaaccaaatgttccagagcttttccaagcctccaaaatgcgcttcagctaaac
aaaacactttatctctgctaaacccttaacccttaaatgtaacatggtattactggcttctttggcagtgtaggggttggctctgttctgcaactgatga
agatgccactgtatgtgccaaagggaactctttaattaataaggagacagaagagaaaggcgatcatagaatacataattgtcagttttgtaaagttaac
aagattattgtaaattatttgatatctggttggtgccaccccttcttcctttctcagaaaaatgtagacctgagacaattgtgtgatttccctttcacac
agggaattgggaaatcattttgcacactttcctaaatgctgaccag
3' EST: CX980540 [+]ttattaaagacaacttgtaaaagaaatcatttcccttaagacttgatacagttctacacatattgaacacaagagtgagacaatattagcagtaattgta
actaaataggaactttaaacagaaaataaatttacatatgaaatataaaaactgcaaacattttcagtatgtgaaatatgctgttcagcaaaaaaaaaaa
catgattgtttttgttgcaattaagacaccagggtttgggagtgttttagctttttgttgtcaggtgaaacattcatttcttgcaccatacctgaaaagg
ttgctattccaaaacttagttaaatgcaattatgtatgttcattgcttaatgaattcttgcagctagaatccctatggttgcccactctgttattacaca
ccatgatatatctatatggcaagtgagcatttaaaggaataggctagaggtgaccaaataaactacaagtcccatcatccactggccatttctgtcaggc
caagggcaaagatttatgaaattccactcctctatggcacctaaaagcctgggtatgtaaaagcacgcattacatctacagttgacaagggtggagcatc
aaaccttaatcaaggcatatggagaaaataatggtttcttaaagaatattcctttttggccacatgcattttaccaattaaaaatatataggcttatcct
gaggaaaggaggaagagactagctcattgcctgatattggaccagttcagtgcagtaacccacagctaccaatcagccacttgctctcagtaatcagtac
acagattaatactgnatcgttagtatggattactgcatttagccagttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: