XB-CLONE-3542711
Clone Name: IMAGE:7718631
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Gene:
ddx21
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980559 [+]
ttatgccgatgaaatggaggttgaaagtgtcaagaagaagaaacagtctgaaactcctctacccaagataaaaaagagaaaaatgcagaatggggcaaca
gaagatcttgatttggaacaggtggcagaatctgtaaatggagagatcaacaacaataatccgactcccaagctgaagaaaaagaagaagcctgtgccac
aatcagatctctctgaaaccgcagaacagtatgaaggagagcagccagatccaagcactcccataccaaagaaagtcaagaagaaaaaactaaaagaggg
caaggaagattcagacacacaggaagggacagacaaatccgaaccacaaatgaacggcgtaaagagtgttaaaaaatctaaaaagaacacaacaggtgat
gataatgaaccaactcctaagaaaagaaaaaaggatacaatggagatcacagcatccgaggagtgtgaagaaaagcagttaaccaaagaggaagaggaaa
taaatcaagaaaaaatagatggcgacttctctaaatttccaatttccaaggacaccattaagaatttgcaagctaaaggcgtgtcctacctgttcccaat
tcaatccaaaacattccacactgcttacagtggcaaagatgtagtggtccaggcccgtacaggcacagggaaaacattttcctttgctattcctttagtg
gagcggcttaatgaggaccaacagccgttggctagaggacgtgcgcctagggtgataattcttactcccaccagagagttagctattcagatcacaaatg
anatccgcagcat
3' EST: CX980558 [+]tcttctcatcagctcctgggcgtattctttaaaatgctcaatcacatcagctggaactgaatccaaggactttattgcatcagcactggatgatttggca
acattcaataaggaaggtattccaacacgtttgaatgtgattcccgtgctcctctccacattccgaagataatgcctttctcttggttcataaagggata
tgcagattcctgtgcgtccggctcttcctgtgcgccctgaccgatgaacataagcatctgcttcctttggtgcagagtacaatactacaaggtcaacttc
tgggatatccaatccacgagcagctacattggtcgcaacaagaacttcaaatgatccctgcctgaaacccttcaagatgacttccctttccttttgctgg
aggtccccatgtaatggtttggcagactgttttaatgagccacagtttgtggctaattcgtgagcttctaattttgaatcacaaaagatgatggattttc
cgtgactaccactgtagacctgaactatatctccaaggaccgctgccttctgggacctggtgcactcaatggccaaatgctcaacagtgatggcagcctt
ctgacttctatgtccaaccaagtcaattttttcaaactgttttttcatatattttttagccacattgtacatccagtctggacatgttgcagagaaaaga
agagtctggggattttcttcaggatcaggtttgtagcgaacagataatatttcctcgacttgttccgagaagcccatatcanacatcatgtcaacttcgt
ctagtaccacatgctttaaaacagtgagg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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