XB-CLONE-3542622
Clone Name: IMAGE:7718591
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Gene:
tbc1d31
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980618
[+]
ccgaggtataaaatgctctttacatgtgaattaaatgcactgctcactagtttaggtccagtttgtttcagagaagtgcaactgagggtattttctgcgt
gcttgggtagcaagtttctgtcggagctctcgtacttctgatatcagggctcgctctctgttctccagctcccctcttcctctgtctaaagaaaagtgag
tggaggagtcgcttgcagagacatcgtttaaacaaacattttcggctacgtgccgctcagcgctgctactgaaatcattctgtgtggaattgcggcttaa
atcgcgaagtctctcaaaatattcatttccatcctctgagatattaacaggttcagcttcctgccttaacctctcctgtaagaaccgattccttttggcc
tgcagcgctgagcttgcaagctcctctgtttccagttgagtcattttctcagagacctctgcccactttcttgtttccatggctttcagaacatcacata
actctcgctctttctctcggacatcgtcagtcattttggacacttcttcgtagcgcttccctcgctcctggtcatcacatttctgtttctgaagggtttg
tattttccagtcggtttccacacacagacgttcagcctttgcaaggctctcttcaagcagcttcttatctttaaggtcttcctctgtttccataagcctc
caaaacatttgttcttccaggtcggcccttctccggntggcatccatctctcctttgacttcctgggtcacagcctcctgctccttagcagttgctgttc
aaaa
3' EST: CX980617
[+]gcgcatcacgacaaggaactgcttcagcatttcataaattatggcgtgacttctcaggtttatgcttggccccttctggaaacccttttctctgaggttc
tgacaagagaggagtggctgaggctgtttgacaacgtgttttccaaccatccgtcattccttttgatggcagtcgtgtcttatatcatctcctcccggtc
gcccctgctgcactgtaaccaaaaggatgactttgagtacttctttcatcaccggaacaatctagacatcggcaatatgatccgagaagcctatcatctt
atggacacatctcctgcagaaatccatcctcggcgcttgctaagtgactttgaacctcttaccagagggcaatatcccatatttaacaaataccccaaat
tcattgtggattatcagggtcaggaacgagagaggatcaggcaggaggaaatagaatacctgcgagagaggcaattgactcatgaagtggaagctgaggc
agtaaagcgcaggttggaagatgaggcctggtaccagcagcaagaacttctcaaaggggcagaggagcagagacggaagcttcttatggacgaggagcaa
aagcttctgcatcagaggcagaggctggcaactgtaaaacgggaactcagactgaaagaacttcagctcctggatgcagccagaaggcggtttctgaggt
accagcaggaccagcgcanaatggaacttagaagactcgacgatgaattagagagaaagatgagtttgcgagaacgggaaactgccaccatagcgaaaga
cgt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: