XB-CLONE-3542533
Clone Name: IMAGE:7718599
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Gene:
fpgs
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980677 [+]
actgaatcccgatgggctgtggccagctgcttaagcagccctctactggacattctgcatcattacaccaaattaataaataggaataaagcaataaata
tattgggcagtttgtaaaactaaaaaaagaaaaagaaacaaaaaacccagtacccaggtacttctcactattgtagaacaaggcacccgcatcaggactg
ttgggaaatagcagcgttagacagacatgcaactacatggcggcaggggtgggggctgtagcaccacaggtgttaaacaatttcagaaaaaaaaaattac
agattagatgctaaaaacgcatcacagaggcctctggtagttaaagagttaacaagcagtggggagaagccctttcattcaagtaaaaatgctttaagaa
ttgccatggcactactgggacagggattggtcgagcagtttcagggctccccctacaaggtgcaggctcccagtaacaagaacttggacccgtgctgctt
ctctcaatagtaccgacccagagctggcgacagggtggtggtggagcaggttaatgtccaggcatgggaattccttatctcttccttgggttgcccactg
cagggcatgagagatacaagggaacactaaggaattgcagttcaaaaagcgctcagaggaaggggtaagaagcagtggagagccacgtgggctgtcttta
atgagggcatctacaggcagggcagaactccatggagtggaacagctgcgttcctcattcagctgattccacatcttttggttctccaggcatcgggtca
ggacattctccagggntacgttgaaattttgctgatctgccattccactccagacact
3' EST: CX980676 [+]ggcgagaggtctctgcccagtgtgtacccacctatcctgcccactggcacttcactaacagtccttctctctctctgcagtgccccctctacatgagtcc
tgaccttgcagagtacgaggatgagggcatcaccctaaagctgggtctgaaaggggatcatcagaaacttaatgcagcattggcattacagttggcacag
acatggcttcagcgccaaggctatccaggttttggggaacaaaagagtccaagctgccaccttcccccaatgaaatggccttttcctttggcgtctgcct
tccgccccagtatccccatggtgcaagggctgcaggataccatttggctggggaggacacaggtcttaaagcggggcccaatttcctactatatagatgg
ggcccataccagtagcagcataaaggcctgcatcaggtggttcagagaggtggcgcttaaagaagaaaaggctggaggaggttctgtggttcgggttctg
ctgtttaatgccacgggagagagggacatggctgccttacttaagcttctgcagccctgccattttgactatgctgtgttctgcccaaacatctgcgaag
tgtctggagtgggaatggcagatcagcaaaatttcaacgtaaccctggagaatgtcctgacccgatgcctggagaaccaaaagatgtggaatcagctgaa
tgaggaacgcagctgttccactccatggagttctgccctgcctgtagatgccctcattaaagacagcccacgtggctctccactgcttctt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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