XB-CLONE-3542310
Clone Name: IMAGE:7718430
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Gene:
optn
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX980833 [+]
tgacattaggcatcacaggtcataacagtaggtgttcacttgtgattttttttttgctaatatataataggataaccccagagtgcaaatcatattagcg
gaactatttttagtaccttgatgtttaaagcaaagatcagagctatttaagaacaaatctgtgtttggtaaagtgcttggtgatattatatatacaattc
taaatgcaaaggactctgtgttgcatttagtggcacaaatggagatcttgtgcttaggtgatacagtccatgacatgaatctgcagcgtatctatatctg
gaacagtcaggttacattttgggcatgtatacacagtaatacttggctgtcccatgttttctgctcctcttggcacaagataaggacccccgctgacatt
ctctaatctgcaactatggcgcctctggagctgttctattgactgcctccccatcatttctgccttaagtttttcactttcttgtatcatatatgccaga
cgagcttctaactgctccttctcttgatgaatgttctctctagctgatctttcggcatggaaatctgcacaatagacgtcaatctgagcacgaagcagac
caattgtttctatatcctccttgtgttttgcgtccatttgtctaatttcatcaatttcaaattgttttttcgccagtgccttttcacatgcatccagctt
ttcaaccagatcgttaaactcaactttggaaactttttcactctcgcgtatccttgtttcctgatnatctgagtttagtttgtcatatgnca
3' EST: CX980832 [+]ggaaaatggacatcaggatgcctcatgtgatgtttttagcatgaagaatgatgcagaaatgttggaacagataaaacagcttctaatggaaaataataat
ctaaaagaaaccatgaagcaaatgaatcaagagatgaaagaacgccttgaagagctcctgaagagacacaatcagcatttgctagacttgaacagcgaaa
atgaattgttgaggaaggaattaaacagtttaagagaaaaaaatgccacatctaatcaaggtataccagtttgctctaacccagaggagacaagtgaaag
caaacagctgaagactcagttaatgcgtttacaggcagagaaagctgacctgcttggtctaatatcagagttgcagctgaaactcagcggtttttctaaa
gactcatttgtggaaatcagaatttctgaaaaggagtctggagaaataatacatgaagaaaaagcaaataaaacactaccagactatgatgtgtcatata
gcaggacaaactctgtcaaggaagaagggagtggcactgaaccagaagaggtggctatcagcaggctccttcgcagtctgagagaggaaactcaaaaagt
ggagagactagagaaggaattaagtgaacataaaaggttggcagaactaaaagaaaaaacctccagattctgtgacaaggacatgcaaacagagcaggaa
aaacagaatcagtctgaagagattatttccagtgaagtagatatattaaagcagaaagtgaagtcactgaataaagagcttcaggaaaccaatgataagt
taaacgnnagctaaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
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