XB-CLONE-3542053
Clone Name: IMAGE:7718360
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Gene:
cirbp
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981009 [+]
cagaggaaagcttggagcaagtgtttagcaaatatggacaggttgctgaagtggttgtggtgaaggatcgggaaagcaagagatccagaggctttggctt
tgtcacatttgagaatcctgaagatgccaaggatgctatgatggcaatgaatgggaagtctgtagatggccgtcaaatccgtgttgatcaggctggcaag
tcctccaatgataggagaggtggttacagaggtggctcttctggaggcagagggttcttccgtggaggcagaggccgaggtggtggtggagacagaggat
atggtggaagcagccgctttgaaaacagaagtggaggttaccaaagcagtggatccagggattactatggcaggagtcatggaagctatggtgatcgttc
cggaggatcctacagggatagttatgacagctatagtaagtatgtggggaagttactagagaggagggtggaaagggtggtacgtcagttgtgatcttcg
tgttccatccagtgatggctcttaacttctcaatttctcaatttgcctctaatgcatgtgttggttctgtcctggctacaggcaataagtgttgggtaga
catagctgggaagggtatgtagtaccagtttaggggaggtgatgctggaatctgtgcctttacaactgtgcctgcttcttgtcctcagctacacaagagt
aaaatccattcctgactcaagatcgtccttccaatggctgtatttataaagatttttggagcttcgccgaat
3' EST: CX981008 [+]atagggcgagaggttttaataggcttcgtgaagccaatcaaaagtagaatacaattccttagccacttcagtacaatagttgcatgtttagtaaaaaaag
gacaatataaacaaacattttaagaacttcttctgatattgttactggagatgcgaacactattcactttgtgtatcaaggaggtgtagccactatatac
agcgcacccaagctctgccttgccttgttacactaaaggaatgcaatttaagtggactcataaaattaacttttcatgtaaccaaaaaatacagcagctt
aaagttctttaaacaatgctacaggaagacaggcctagcagtctgctactaatacaggtgcttctctgcccatcaaccaacagcacccatccgcttagga
agttacttcctagcactacttaaccagaagggggagcagccccacctgatcagcttgaatggaacagcatagtaggataggaatatccttgcagaacaca
gtaactttaagaatgagattaggtgtccaaagataaagcctttgaaacacagctgtcccacttattagagaagctgtcaggctaactaggtctattaaaa
aaaaaagtgaacacaagtagatatactacacaaacgattcggcgaagctccaaaaatctttataaatacagccattggaaggacgatcttgagtcaggaa
tggattttactcttgtgtagctgaggacaagaagcaggcacagttgtaaaggcacagattccagcatcacctcccctaaactggtactacatacccttcc
cagctatgtctacccaacacttnatgcctgtagccaggacagaaaccacacatgcatttagagcaaattgagaaattg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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