XB-CLONE-3542050
Clone Name: IMAGE:7718408
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Gene:
gpld1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981011 [+]
ctttgaggtcactgtttattccacactattgttctacaaagctagtgatggtgacatgcaaagagctaactatacaaagctagtgtaggcatgtatagaa
gccactattactggtccaaaacaactgggcgctggtaaatgacataggatagacaatataatgtactgttgccctgccctggtaaaactgatgtgtttgc
tacagaaacactactatagtttatataaataagctgttttgtagccatgggggcagacatttatggtggaaaaaaggagaaaaggcacaggttacatagc
agagagcagatattttttatatccctgcattgttcacacctcaggctcaggccgtaataacccacattgttcacctgttcacacctcagccagactgtag
gagcagtgtcagcattgtgtcactgaatgctgccttgttgtgccatactctgcctgccctatgctgcctgtgtgtgccatactctgcctgccctatgctg
cctgtgtgtgccatactctgcctgccctatgctggctgtgtgtgccatactctgccagccctatgctgcctgtgtgtgccatactctgcctgccctatgc
tgcctgtatgtgtcatactctgcctgccctgtgctgcctgtggggggtgatcctggcaggggtgtgttctgggagattgttaacatgtggaaatagtgat
tatatggtccctaaggtgtgtaactatgtgctgggtgttgctgtgctatccacaggggagacatatggatttaagggtatgtcataatacacttcttttg
aatatgagtgaagggtgatatccctgtatccc
3' EST: CX981010 [+]tgggtagagcagcagctgatacaagatggaacagagcttaaagataccatggtaatgtgagttcatttttaaatagttgccattgaaattattattttag
gatgggccgccagtggcacttccaactactcttagcagggtgctggaaactgtagtctggattggccattcctgtactgaaaagcaatcatttcctgctt
tcttctttttactaatcttttatattctaaaataataattatacaaccacatcaggttaaaatacaataaatgaagacgccagatccagaatgcagacaa
tggtataaaacttcctatctttgagaggagtcttatgttatgtgttattcagtcatgtcttttgtgctgcaacattccctctcctgtctttttgggacag
attattcatttacatcaaatgctaacttgccccactcttcaacccatggaactgcacttcattattcatgacattatagccagctatggaccaaactggt
aaaatctcttggtctatctgcagtaccaaattattacactaaatgtttacggatgtattacatgtattatttcataaaacattagtcaaactatttggca
aactaccaaacaatttcctgaatatgatgagtaaagttctatattatatgttgatgggcctgtcctctccagaaaatatgtactaggaaagacaaaagaa
caattgctctggtcatttatgtgtataagtgtcagactgaaatgtaaagctgctttacactagagatctagggccatatgttgctgtaagaaagctattt
acatgactatatactgtgcacaggcaaagcgggaagttatgcaaaaatgtagatatggagggcactccaagtcttgctttcgctgagcttctttcagcag
ctacaacaacttgact
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: