XB-CLONE-3542008
Clone Name: IMAGE:7718412
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Gene:
decr2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981040 [+]
tctgaactccccactctcgacctgcccccagaggacatagagacagatgattgtcttctgtcttacaactacttgtacagccccactctgctcaaaggca
gggtggcatttatcactggaggggggtctggaataggattcagaattgctgagatatttatgagacatggctgtgacacgatcattgtcagccggaacct
gcagcgggtatcagaggctgctgaaaaactcaaagttgccacaggccagcgctgtctgcccctatccggagatgtcagggatgcccagagcatgaatgct
gctgtagaagaagctcttaggatattttctcgtgtcgatatcctggtcaacaatgccgctgggaattttctctgccctgccagctctctctccctcaacg
ccttcaagactgtcattgatatagacacagtgggcactttcaatgccagtaagatcctgtttgagcgatttttcagggacaatggaggtgtcattgtgaa
catcacggctacactgagcttcagaggacaggtcttgcaggtgcacgcgggcagtgccaaggccgctgtagatgccatgactaaacacctggccgtggag
tggggtcccagcagagtacgagtgaactgcttggcaccggggcccgtctctggcacagagggtatgcgcagacttggcggtgcagcagcggaggcagcag
gcgtttgggccactcttccacttcagcgcattggaaataagacagagattgcccatggagctctcttccttgccagccctctggcctccttcgtaactgg
cacaactctggtgatggatggcggatcatggatgacat
3' EST: CX981039 [+]gaaaatacaaaaaacattttaattagcagccaaattacaaatgtaggcacaccatacaagcgtaacccctaattacaaagaaatagtatcattgctcatt
gccctttgccctggcacagttctgttcgatgccactccacatacagtggccaagtaatggcttgtacatccattttccaagtgcacaaaatgctgattat
ttgtgttgttcggatactcatggaacaatgatatttgtaccagtacagctacggccaccattagtgttctgcctcggctggaatgttacgggaagagcca
aatgggcaacttgatcagttttattcgtccaatgcttcatttgtccttcttcagcccagaggcccaaagatctaaaggtgaggagaaggacgggtaccca
gcgatggtgccagatgattttgtgatgtcatccatgatccgccatccatcaccagagttgtgccagttacgaaggaggccagagggctggcaaggaagag
agctccatgggcaatctctgtcttatttccaatgcgctgaagtggaagagtggcccaaacgcctgctgcctccgctgctgcaccgccaagtctgcgcata
ccctctgtgccagagacgggccccggtgccaagcagttcactcgtactctgctgggaccccactccacggccaggtgtttagtcatggcatctacagcgg
ccttggcactgcccgcgtgcacctgcaagacctgtcctctgaagctcagtgtagccgtgatgttcacaatgaca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: