XB-CLONE-3541855
Clone Name: IMAGE:7718618
|
Gene:
adgrf1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX981145 [+]
tattaacctaactggaaacaagagttacacgtgcacatcgacgggctggcagggcgtagtgcccgataactgcgtactgcaagttctcaacagattagtc
tctgatgtcccgagtctgacaggcccagggggggcagagatattgcctgtgtatgttgcaaaccttagcgccacagtgacggcagagaaggaaaacataa
cgtcttcaccaagcaacatagtgacggtagtcaacatactacagcaagtgacgaccaccactcaaactgtacagccggacgttatgcagaactttctgac
gacggtcaatgttgttgtagatgacacatctaaaaacgcttggaataacatgaacaataggactgataggggtacccagctgctgaactcagtggaacaa
tttgccagaaagcttcaaattgacaacggatccatttcaataataaacaattccaatgttcagctggctggcattattgcaaattcatcaagaagctaca
acgaaagcttcaatttctcccaaggcaataacttgacaggcagtgttcttatctccaaggatagtgtgagcaacatggagaataataccaacatcattac
cattgcctacgcaaccttgaaggacattttgctgaatacgtcaactaaagttgtgaatggcctagtcatgacaacggtactctccacccaagtgccttcc
aattttaaaatttcaatgaactttaagaagagcaatcagacattgaatgttccagggtgtgttttctggaatctgggaactaataactgggacagtactg
ggtgccaag
3' EST: CX981144 [+]atagggcgagaggcacataagtaataaaataaataaattacatgaggaatatgattaaacacctacacgtgtaccctaaagagtatgcgtctatgcaatg
cttatgtctgttatgtgaactcaacgcggtatcaacaaagcaagactttatcctcagaaccaaggacccaggagtccgaccagttctttctaactctttt
aataacttaggactttgttttaacataaaaaaccccatatagacatatcaacaggtgttcaggtggtagcgcataaaaaccaggctgatgaacttcattc
agtttgaattagaaccagcaaatatctattttgtccattactccagcctgcaaggatctaggaatatccctcgttgtatccaggtggctccgtcataagc
tgcgaggaaacatttaaaacaataaaggtgcttcaattgctccacctgagtgttcagaaggacatccatacagcttaggggcggtgtatgtgcagggact
ataggaatcttctctatctactgctcagtatactgcactctaagagaatgttccttcccagaaggaggcagaataaaactggtacggaacattataacac
ttacattataggctttttacacaataataatcgctaatgaacatgttattctagtgtaatcaaatatgtatttcctgtttgtgtaggagaggcaaacagg
gtttattaactgctacggttactaaattgggtttgaattgngtgatncaggatttagctgaatcccaaaaatgtaatatatattagataagactaaacat
ggggagaggggtcaaggtgg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: