XB-CLONE-3541849
Clone Name: IMAGE:7718715
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Gene:
eef1g
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981149 [+]
ccgaggcggtttgctgtcttgttaagttaaaaggctctggatgttggtacggatgtgaaaaccagactctgcgcgtattttagggggacagagatcacct
cctcctcatttgaagatcttgccctggttaaaggcctttcccacgtgcttgaagtctccctcccagcagaagtattctttgaccagggttttgcactcct
cactattgctgtccagctttctccaggtgtaggattcatagtcaatttgccagtcttcagagagtgtgaatgcaaggtcctggcccctgaacacccacac
gccggagatggagctgtcattgttggtgccaaacaagatgacgctggcaaagccagtcttgcgcagtttgtctagacgctggaacatacctgtaattaag
ttgcagctcatgaaggtttgtgtcagctcctcggggaatttgtattcagcgtaccagatggaccagccttccttgtcaaagtgctcccagaaataaggca
gagctacagccagggtatcctcattagagtattttctcttaaactcatccattacaaaagagcttttaggtagatgtgcgtatgggtcctttgatttagg
ctctgctgctaaagccttttcagattcatccatatcatcctcaggggctggggcaggagcaggagctggtgttgccttcttcttttcctccttttctttt
tttggctcctttgctggcttctctttcttgggagtttctttcttgggctgcatctcggcaaacttcttagcatcaaactgtgccatcttgtcacaaagct
tgacttctcccacacagcacggaattctggctg
3' EST: CX981148 [+]gctcagtattcggggttccccatcaaggttgcctcctctcctccagaattccagtttggagtaacgaataagacatcagagttcctaaagaaattcccct
taggcaaggtaccagcatttgagggcaacgacggtttttgcctttttgagagcagcgccattgctcattatgtgggcaatgatgagctccgaggaaccaa
tcgtttacaccaagctcaggtcatccagtggctcagcttctcagacagtcacattgtccctccggccagcgcatgggtctttcccactttagggatcatg
caatacaataagcaggccacagaacaagccaaggaggagataaaggctgtgctgggtgttttagattctcatctgaagacacggacattcctagtgggcg
agagggtcacgctggctgatatagcagttacttgctctcttctgtggctctataagcaggtcctggaaccatccttccgccagccatttggtaatgttac
aaggtggtttgtgacctgtgtgaatcagccagaattccgtgctgtgttgggagaagtcaagctttgtgacaagatggcacagtttgatgctaagaagttt
gccgagatgcagcccaagaaagaaactcccaagaaagagaagccagcaaaggagccaaaaaaagaaaaggaggaaaagaagaaggcaacaccagctcctg
ctcctgccccagcccctgaggatgatatggatgaatctgaaaaggctttagcagcagagcctaaatcaaaggacccatacgcacatctacctaaaagctc
ttttgtaatggatgagtttaagagaaaatactctaatgaggataccctggctgtagctctgccttatttctgggagcactttgac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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