XB-CLONE-3541684
Clone Name: IMAGE:7718969
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Gene:
amy2a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981262 [+]
catccattgtgcacctgtttgaatggagatgggacgatattgcagcagaatgtgaacggtatttgggaccaaatggctttggaggtgttcagatttcacc
cccaaatgaacacattattgtgacaaacccttatagaccttggtgggagaggtaccagcctataagttacaagctctgcagtcgttctgggaatgagcaa
cagtttagagacatggtgacaagatgtaacaatgttggcgtatatatttacgtggatgccgtaattaaccatatgtgtggctcaggtggaggtgctggga
ctcattcatcttgcggcagctacttcaatgcaggatccagggagttcccagttccctattctagctgggattttaatgatggaaagtgcaggactggcag
tggtgaaatagaaaactattcggatgctaaccaggtccgagactgtcgtcttgtcggtcttcttgatttggcactggagaaagactatgttcgtggaaag
attgctgaattcatgaacaatcttattaacattggggttgctggattccgactggatgctgctaagcatatgtggcctggggacctaaaggctatttcag
aaagactcaataatctcaatacaaaatggttccctgctggagctcgacctttcatcttccaagaggtaattgatcttgggggtgaagcaatttctgtcaa
cgaatactatggaatcggacgagttactgagttcaagtatggtgccaagcttgggacagtcatcccgcaatggaatggagaaaagttgtcctacctaagg
aactggggagaaggctgggggatcatgcctaatgac
3' EST: CX981261 [+]ttagggtagctcacttcagtttattaggtaattgcattttagaaaatttgtaaatagtttttttcttaggtcactgcacttaacatagtgcaggggtata
ttatatgagatcatgtttttggaactttataatttggcatttacatgaatagcagcgaatgggtcttcgtcgttgttgcttatttggaatttagcatagc
cattgccatccacattgatctgcctgcctgtgcagcgagatccctccttctgtccagagatcacatcacagtaggttccacttggaaggccagtattcag
agtgacatccaattgccaatcatcattattaaagattatgaatcctttattgccacgtccaaatgcaacttggttgctcccattatcccaccagtttgag
aagggttgaccatctgctacattacggaaggtaaccatgttcttaatctgacgccatctatgctcacagacccagttgtcaccacatgttgagtcagggt
tgattgggactgatttggtggatccatcggaattactaggagggccaatccagtcattttgatcctttccattgactatgttcctagaccatctgaagct
agacatgacacgagtaaaaccataaggatgtgccagcatgaagccaactcccattttgtacagacgtgcatcccagaaggtgagtatggaagctcctcca
gcaccgtgtcctctctgattatcatgattgtcaacaaacaccaaggctctgtcattaggcatgaatccccagccttctccccagttccttaggtaggaca
ac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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