XB-CLONE-3541666
Clone Name: IMAGE:7718875
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Gene:
cldn5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981274 [+]
agacttgattgaatataatattaactgtagacaaataaaaaaataaagcaaagaatgcagtggctttttttaacagtaccaaagaaatccagccctgcat
tatacagaatggtcaacacagaaatatatccagttaccttcatggcagtgtcaggagcgatgggggacatccctgccaaattgttgcgcagagtgagttt
aacagatagctaacctgcagctgggcactggcagtttgaatataacaagagtttgcaaaactagagtaattttactctggagtagttgccactagcaacc
aatgataaatgaactgttgaaattctaattgatggcaatgggtttctactctagataaaattgtatctgctttctcaatgtaccccaccccaaatggcca
caagctggatcatccctggccattatcatgaaattaatcattctatggtgatgtattatgtgtgtagccttggaacattgggagactattacttatgcta
tttaggggtacaggattgaaaggccagcaccctgaaacaagggctcttgtgcaagggcatgctcctcctttatagcagtgtcagcaaaaggcaacagctt
gctatgtatttatatattttattatattagcagaaccggggtgataatagataaactggttatggcaatgcactacaaggttctgtagcacttcaaagat
ataaaggcattttctataaataaatcagacagttaatttttccaatcatttcctaaatgggcagagatatagatgtacattgtatctctctctatttgcc
ctgctttccccagcagtggatttctcctggggt
3' EST: CX981273 [+]gagacatgtatttagtcgaagtgcagacatttaaatgtataatattagactgtgtttaagggctcttacacaagtgctttaagttgtgttcagggcttcc
atgtgtctgaatgaatagagccacgtaggaaagaattgactgtttcatcctgcactcccctgtggtggaacccaggagaaatccactgctggggaaagca
gggcaaatagagagagatacaatgtacatctatatctctgcccatttaggaaatgattggaaaaattaactgtctgatttatttatagaaaatgccttta
tatctttgaagtgctacagaaccttgtagtgcattgccataaccagtttatctattatcaccccggttctgctaatataataaaatatataaatacatag
caagctgttgccttttgctgacactgctataaaggaggagcatgcccttgcacaagagcccttgtttcagggtgctggcctttcaatcctgtacccctaa
atagcataagtaatagtctcccaatgttccaaggctacacacataatacatcaccatagaatgattaatttcatgataatggccagggatgatccagctt
gtggccatttggggtggggtacattgagaaagcagatacaattttatctagagtagaaacccattgccatcaattagaatttcaacagttcatttatcat
tggttgctagtggcaactactccagagtaaaattactctagttttgcaaactcttgttatattcaaactgccagtgcccagctgcaggttagctatctgt
taaactcactctgcgcaacaatttggcagggatgtcccccatcgctcctgacactgccatgaaggtaactggatatatttctgtngtgaccattctgtat
aatgcagggctggatttctttggtactgttaaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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