XB-CLONE-3541585
Clone Name: IMAGE:7718835
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Gene:
anxa13
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981330 [+]
ctctccttgatcgtctggagatcaatctctgctctggttaccaggatgcggatcagcattgcctcatttgtacctgctcctttcattgctttgtacaact
tcttagcaaagtagccttggcaatcccttgtcacttgcactatggtggaataagctttcttcagatccccggaggtttcacttttaatcacgtccaaaat
gtcttttccatgcagaatctcataggctttaaatgtggccctcagctgcatgtagttccttgttgccaggataatattgaactctgattcttctgtgccc
caccgagcctctccagcctcgtagagccttttggcatcctgaccggcaagatcttcattaatagacagaccctcatcccgattggcctggagcaatgaga
tgagaatttttcgaaagtatccacttgtctcactcttaatgtctgactccaaatccctgtcaaacaatctcttataggcttcttttgtggcttttatttg
ctggttggacctagtacataaaatctgtatcaacaaggactcgttggttccagctcccttcatggcgctcctgagctcgcgggcatcgaattcacatggg
cggtccaacagcgctaatgctgtcttctcaaagttcccgctcagctcactctttagaacactctccaaatccttgccatataacgtcttgtatttctgtt
tcaattcttgcctttggtctgaagtcctgttggccagaatctctatgattgctttttcatctgttcctagacctttgcatgccttatatattttctttgc
atctcgttctgcatcaaaatcatgatgaagcttaatggttggat
3' EST: CX981329 [+]gaaaaatgggcaattttcatccaaccattaagcttcatcatgattttgatgcagaacgagatgcaaagaaaatatataaggcatgcaaaggtctaggaac
agatgaaaaagcaatcatagagattctggccaacaggacttcagaccaaaggcaagaattgaaacagaaatacaagacgttatatggcaaggatttggag
agtgttctaaagagtgagctgagcgggaactttgagaagacagcattagcgctgttggaccgcccatgtgaattcgatgcccgcgagctcaggagcgcca
tgaagggagctggaaccaacgagtccttgttgatacagattttatgtactaggtccaaccagcaaataaaagccacaaaagaagcctataagagattgtt
tgacagggatttggagtcagacattaagagtgagacaagtggatactttcgaaaaattctcatctcattgctccaggccaatcgggatgagggtctgtct
attaatgaagatcttgccggtcaggatgccaaaaggctctacgaggctggagaggctcggtggggcacagaagaatcagagttcaatattatcctggcaa
caaggaactacatgcagctgagggccacatttaaagcctatgagattctgcatggaaaagacattttggacgtgattaanagtgaaacctccggggatct
gaagaaagcttattccaccatagtgcaagtgacaagggattgccaaggctactttgctaagaagttgtacaaagcaatgaaaggagcaggtacaaatgag
gcaatgctgatccgcatcctggtaaccagagcagagattgatctccagacgatcaaggagag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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