XB-CLONE-3541477
Clone Name: IMAGE:7718797
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Gene:
rnpep
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981401 [+]
tttttgatgtttattttggtttattgcattttcagtctgaacaagtgtggtacagtatgttgattacacttccattctgtagctggacaatgatctgcaa
tgctctatatgcgctctcattgtgcagatgtgcaactctatgttcctaggatcctctttacatagttctgcacattggcatgaagctgtggacaagtttg
cgtaaaggtctccctagcgaggtcctgtgctgcctttgagccagcttgcatggcacggtataaaggtaaagtatacttctgcttcccctggcaatggaga
aagtccctgactttatgaaagtggagctgatagtcattcttcaatacaatctgggcccagcgcaaacgcagctcagcatttgtagcattggaaatctttg
gataatacttttccagttgttcgacatttccatcaggcagtggtgacaactcaagtactctatccaaaaagtagacaagctgataggtcctccatattgt
gggatcaacttttgaaatagtttcagtatcaagtggagtagaagaccagaggtttgctagttctgttgctggtttcataagtgcctctccaggggacagg
tctgggaggaaaggaggccatcctggagtgtttagccaatggtcaaattccagaccggatatcgtatccactccctttgctttcagttctggaaaatatt
ctagataaaactcaagggcttcatcagcaagaatgctctggaacttgaacttattcacataggcctttaaaaatgcatcaaacttggtctggtccccagt
gaggtgggccaagtatgaaacaaagc
3' EST: CX981400 [+]gagggcctaagcagcgcttagagacctgtaattgtacagcgtagcgggacaccatgtcagccagaggcaggacggcggaagatgtagccacagcttccag
ttaccgggagttccagcccgagcacatgcacctggagctgacagtagacacagaggctcgaatactgcggggctcccagcggctggacgtgcaggtgttg
cgggagaccgaggagctgcgactggacactcaccgctgcttgcgagtgctgggagctcggctggagaagggccagggggaacatgaggagttgtcgtggg
aggaaaagcctttcacacgctatggcactatggtggttctccgtctgcccagcctactgccccccggagccagggttcgggtgatactcgattatgaggc
agcggatagcccaggggtttgttggttggacccacagcagactgcaggcaagaagaagccttacatgtacacgcaaggccaggctgttttgaaccgctcc
ttcttcccatgctttgacacaccggctgttaaatgcacctattctgccaatgtcaaggtgcctgaaggttttacagctgttatgagtgcaaataactctg
accgccaaggggatacttttaaattcgagatggcacaacctattccagcatatctggtggctctggtggttggtgacattgtatcagcagaggtaggacc
aaggagccgtgtgtgggccgagccttgtcttatagaagcagctaaaaaggagtatgatggagtaatagaggagttcctganagtgggggagaagctcttt
ggtcctatgtatggggaggtatgatgtactattcatgccaccatcttt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: