XB-CLONE-3541468
Clone Name: IMAGE:7718941
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Gene:
cpb1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981407 [+]
ctaagattaaaaatattaaatacaaatggacatcacaagacctttagcactaagttagctttgaataacttcatttagcattgttctttatgtagctggc
aatgtatttgacggccagcatggtttcttcacaggtaggtttaatctgagattcagggagggcaaaaccatatcgtcctgtgtcacggagctcaaatgtg
tatgagaatttaacgcctgcgtcgtaggcccagtcatctgatccacctgctgcaaggtagattgttgatcctccgggaccgtaagtgtacttggttttgt
acagtgaagtcagactgttaactgctccctgagcaactgcattcagttcattgtgatctttagcaactgcataactgtaggaataggggaacagcaacat
ctggctataagaatggatagtaaggtaacccttgattgcaggaatgtttgcgcggatgaagttagccaaagcctttgtctctggttcagattcaggagca
gagccacagtatgtttcatcacaggctcttgttgaggctccagcagtgcaccagccagcattaaagtttctgtttgggtcagttccaatgcaagtgctgt
tggggttagctgaacgagtcttcctccacatacggttagtggtccaggtgtagacgtaaccatcaacattcaatactggcaaaacatagatgtccaggtt
gtcgagcagagaggtaaactcagattcaacgccatatgcactcacagcctctttaacaaccactggcagaaagca
3' EST: CX981406 [+]agggcgagaggaaaagtgttccgtgtaattccacaaaatgctgaacatgttgaacttatcaaatctatggcccaaacagaagggcttgatttctggctcc
ctgactcagcacaacttgtagaacaaggaaaacgagctgattttcatgctgatggtcatgtctcatatgaggttcaggcccttctccagcaaagtgggat
gccctatgaaatattgattaatgatctgcaagatgccctagaaaagcagcgcgacagcaacatcagggctgttcacagttatgagaaatacaatgatctt
gatacgattaatgcctggtctgccaacattgctgctcagaacccaggacttgtgtctcgttcctcgataggaacctcatatcaaggccgtcctatttact
tgctcaaggttggaaaaagcggtgcaaacaagaaagctgtattcatagactgtgggtttcatgctagggaatggatctcccctgctttctgccagtggtt
tgttaaagaggctgtgagtgcatatggcgttgaatctgagtttacctctctgctcgacaacctggacatctatgttttgccagtattgaatgttgatggt
tacgtctacacctggaccactaaccgtatgtggaggaagactcgttcagctaaccccaacagcacttgcattggaactgacccaaacagaaactttaatg
ctggctggtgcactgctggagcctcaacaagagcctgtgatgaaacatactgtggctctgctcctgaatctgaaccagagacaaaggctttggctaactt
catcccgcgcaacattcctgcaatcaagggttaccttactatccattcttatagccagatgttgctgtccccctatttct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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