XB-CLONE-3541124
Clone Name: IMAGE:7718876
|
Gene:
etnppl
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX981636 [+]
caaaaacttcccagccacagcagcagagatgtgtgaggaggagtactgcaaggaaaagatcattgagatgagaaaaaagcacattgggccttcgtgcaaa
gtgttttttgcaaatgaccctatcaagattgtgcgtgcaaaaggacagtacatgtttgatgaaaaaggagagaaatacttggactgcataaataatgttg
ctcatgttggtcatagtcacccagaagtaaccaaggctgctttgaaacagatggaattgttaaacaccaattctaggtttctacatgacaaccttgtccg
gtatgctgagtgtcttacagccacccttcctgagaagctttcagtctgctattttgtcaactcaggctcagaagccaatgaccttgcactgcgtttggca
cgacagtacagtggccatcaggatgtcataactcttgataatgcttaccatggccatgtaacctccctaattgatattagtccctataaattccaacagc
tgggaaaggatgcacagaaggagtttgtacatgtggctccttctcctgatacttacagaggaaagtacagggaagatcatacagaccctgcattagctta
tgctaaagacgtagaggacatcattaaggaggttcataagaacaagcgtcaggttttgctgacgttgccctgttgcagaacaagctgtgctaaccccacc
cactttggaaagtgccatgatgttacactctagtgcatctcttcgaatatagtattgctgcttttattgctgagtccatgcagagttgtggaggtcaaat
aatcccaccagcaggctacttccagaaagtgtc
3' EST: CX981635 [+]tgatgttatgtagtaccattaatacttgacattgatttctgttgtctaatatttaaaaactatgactgaaacactcccaatctgtgactttcagtttcac
caatttcctatgccaaacactcaaatatctagatttaatggtcaagaacaacaaaatccaaataactgcaagactggataaaaacatctaaaacacacag
aaacaaatctagtttatttgactagatgtgcagaatttaatgaataagcttttatttcttattatgttttgaatctagtttatagagaacaaatgatata
ctgtagatatgcatggcatggatttagccaagcagtaggatctggtataatgtgattatggatatctgctcttctggcactctttcatagtcattcttgt
caagtgctgttatacatctgaaatgttctgacactttaagaaaagaagcatacgcatgtttacctagttttaagaaaactgctgatcccccatgtagctt
ctcattttaaaatataaaacattatagcaccttgtatgttgacaaaaaagaaaataccataggcagtgagaaaatgcccccttatgctattctatggggc
tcatattcaagtaaaagggatataggaatcatgtacacagggatttcaggctgctacatcagatctgtagggacccctaataaaaagtaaaataatgtag
cgagtggaaaagggttacttatttgagcaagagctaaaatcttggtgtatctaaacagttggaaacggcttaaaattttcttgtacatagcatgctcagt
acattcagcatgctgaggtcttttaaactgcatcttaaatctaagccattaaaacatgngaaaaagattcccattcccatatgatatactggaactgcaa
atacngaataaaaaaaataaaaaagatg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: