XB-CLONE-3541106
Clone Name: IMAGE:7718782
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Gene:
clu
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981653 [+]
ctgacccaggagggagagcagcagtctatgaccctggatgaccttgaggggggctacagtctggtggaggacagtgtcgatgagctcttcagggaaagca
tcaaagcctttgggcaaatgaagccgttcttcagtaattccttccatagcgacttctttgggtctcagtggaacccatttcccttccagcgagtcgggtt
cccatttgctgagtctcggcgggcaaggtctccctcgttccacccctatttctctggggacttcgagtccctcttagaggctgcacagaaaatgatggaa
agaagtcaccactttgccccaaggctggaaggactgggctccataaggggaaatggcgatgaatccgatgacaaactggtctgtaaggagttgcgtcgca
attcagccggctgtttaaagatgcaggaaaagtgtgagaaatgcaaggagattttagcattagattgttccgggaaggatccggtgcaggagcagctgca
ggaggtgttccaggactccctgcgcctcgccgagaggttcaccagggagtacgacgacctcttggaacagttccgggagaagatgctcaacaccaccggt
atcttggagcagctgaacaagcagtttgggtggctttccaaattcaccaacatgacccagggcagaaagaatgggatcttccaagtttccactatctatt
ccaaggaaggagagtctccttccgacaccaaagtgaccgtcaacgtcttcgactccgaccaataaccttcgctttgcccggatacatctccatggatgac
ccgaagtttggggaactggtggccgaggaagcgctta
3' EST: CX981652 [+]tacaggccatttatttgtcggaatcctacaaaaataataaaaaaagcagcacgtggcagtaacagtagtaacggaacagtatgaagagtatcgggctctg
gcgtcaggaggtagcggaggtatcaatacgctttgcctattgctcgggggcgttgccataggggtcgcggacctgttctacggaagtggcaggaagaagc
cgccgatggggccgaagcaaatcccaccggtgtcgggtcaggcagcctcaacagcttccgctttaaacctcttaagcgcttcctcggccaccagttcccc
aaacttcgggtcatccatggagatgtatccgggcaaagcgaaggttattgggtcggagtcgaagacgttgacggtcactttggtgtcggaaggagactct
ccttccttggaatagatagtggaaacttggaagatcccattctttctgccctgggtcatgttggtgaatttggaaagccacccaaactgcttgttcagct
gctccaagataccggtggtgttgagcatcttctcccggaactgttccaagaggtcgtcgtactccctggtgaacctctcggcgaggcgcagggagtcctg
gaacacctcctgcagctgctcctgcaccggatccttcccggaacaatctaatgctaaaatctccttgcatttctcacacttttcctgcatctttaaacag
ccggctgaattgcgacgcaactccttacagaccagtttgtcatcggattcatcgccatttccccttatggagcccagtccttccagccttggggcaaagt
ggtgacttctttccatcattttctgtgcagcctctaagagggactcgaagtccccagagaaataggggtggaacgag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: