XB-CLONE-3541004
Clone Name: IMAGE:7718744
|
Gene:
XB5769266
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX981723 [+]
ctttaatctgcccccctccggcccaaaatgtaaataattaatactgccatatttacattataatgcatattcatcatattgtatatgagaaacataatgc
aatgtaatttatctaatcttggcacagagtttataagggtgtgggatctgcactatataaacaataggatcatcccgtgggcaaggctctccgtcaggga
tctgaaacgagaagcgctgcaggagagacgtgaagaagagaaaaagttccatcctcgccaacctctcccctaggcaagctcggcgacctgtagaaaatgg
taaaaatgcttcttgtttcacaaatttgccatccgcatccaggaagtgctctggatagaactggaatggtttctcccagactttttcatctttaagcact
gaggacaggttggggcagacagcaacaccctttggtatggaaaagccttggacttccgtgtctctgtatgtcatatgaattaaagacagaggcacaaggt
ctgcgcaacgttggatttcatggattactgcatttgtatatgacatttgcaaaacatctgccatcacaggcttcctgctttttccaatcaccttgtctat
ttcctcttgaacctttctctggacatctgggtattgcaacatgtacagcagcccccatctcagggtggtggttgacgtttctgatccagctgtaaataag
tcaaatgtagtanacaacagattcttctcattaaaatgattctctttgtctccttttgccttttccatttgaagcgtaaaggcatctatgagatcccttg
tgcatgcaggattccatgttttctgatggtca
3' EST: CX981722 [+]gttccaaaaattgttaaatctgaatcaagcaatgtttgaagttgaatccggaactatggcccagattgctaccgcaattccctggctagcaaaacttcca
ggtctggcaaagatgatttatcggcctcatgtagatgtgcttgaatatctacagaaaattatctctgaccatcagaaaacatggaatcctgcatgcacaa
gggatctcatagatgcctttacgcttcaaatggaaaaggcaaaaggagacaaagagaatcattttaatgagaagaatctgttgtttactacatttgactt
atttacagctggatcagaaacgtcaaccaccaccctgagatgggggctgctgtacatgttgcaatacccagatgtccagagaaaggttcaagaggaaata
gacaaggtgattggaaaaagcaggaagcctgtgatggcagatgttttgcaaatgtcatatacaaatgcagtaatccatgaaatccaacgttgcgcagacc
ttgtgcctctgtctttaattcatatgacatacagagacacggaagtccaaggcttttccataccaaagggtgttgctgtctgccccaacctgtcctcagt
gcttaaagatgaaaaagtctgggagaaaccattccagttctatccagagcacttcctggatgcggatggcaaatttgtgaaacaagaagcatttttacca
ttttctacaggtcgccgagcttgcctaggggagaggttggcgaggatggaactttttctcttcttcacgtctctcctgcagcgcttctcgtttca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: