XB-CLONE-3540989
Clone Name: IMAGE:7718984
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Gene:
fth1.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981733 [+]
ttttgcgccaccagcaaacaccgaaacgccgccatgcaatcccaggtgcgtcagaacttcaacagcgactgcgaagccgccatcaaccgtatggtgaaca
tggagttgtatgcctcctatgtctatctgtccatgtcttactatttcgatcgtgatgacgtggcactccaccatgtggccaagttcttcaaggagcagag
tcatgaggaaagggagcatgccgaaaagttcctcaaataccaaaacaaacgtgggggccgcgccgtccttcaggatatcaagaaaccagagcgtgacgaa
tggggtaacaccctggaagccatgcaggccgctctgcagctggagaagactgtgaaccaggctttactggatctgcacaagctggcatctgacaagactg
atcctcatctctgcgacttcctggaatctgagtacttggaggaacaggtgaaggccatgaaggagcttggagactacatcacaaacctgaagcgccttgg
ggtgccgcagaatggcatgggcgagtacctgttcgacaagcacaccctgggggagagtagctaagcgcactctcagggtggaaacaaccagtcagctctc
tgtttcaaaatactgtctctttactatctgtgtggattacaccatagctcccatgcccctgttgacacctttttatcttgaggatgatgggaataaatct
gtgctattactgttctgaatcatgcagaagctgctgtgcttgtcaacatgttttca
3' EST: CX981732 [+]tgaaaacatgttgacaagcacagcagcttctgcatgattcagaacagtaatagcacagatttattcccatcatcctcaagataaaaaggtgtcaacaggg
gcatgggagctatggtgtaatccacacagatagtaaagagacagtattttgaaacagagagctgactggttgtttccaccctgagagtgcgcttagctac
tctcccccagggtgtgcttgtcgaacaggtactcgcccatgccattctgcggcaccccaaggcgcttcaggtttgtgatgtagtctccaagctccttcat
ggccttcacctgttcctccaagtactcagattccaggaagtcgcagagatgaggatcagtcttgtcagatgccagcttgtgcagatccagtaaagcctgg
ttcacagtcttctccagctgcagagcggcctgcatggcttccagggtgttaccccattcgtcacgctctggtttcttgatatcctgaaggacggcgcggc
ccccacgtttgttttggtatttgaggaacttttcggcatgctccctttcctcatgactctgctccttgaagaacttggccacatggtggagtgccacgtc
atcacgatcgaaatagtaagacatggacagatagacataggaggcatacaactccatgttcaccatacggttgatggcggcttcgcagtcgctgttgaag
ttctgacgcacctgggattgcatggcggcgtttcggtgtttgctggtggcgcanaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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