XB-CLONE-3540908
Clone Name: IMAGE:7718896
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Gene:
cela1.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981789 [+]
attagatacagtatttacttgcagaaactgattgacatcaacggcacattccttcttccttaaatgcatttcttactcttgtctgtgttccctttccagt
catttagatgttattattgatccatccgatgtaagcagaaactctagtgaatacagttggcttcaggtatgttgagcaaccggaggaggagacaaagctg
gtgacaccatggacttggtacacaccgttgacagggcagttgagagggccaccagaatcaccctggcatcctgaacggactccatcaccaccagcacaca
ccattgtggacttgactgtacttccccaataggagccgctggagcaggtggaatgggctatcacgggcagaggggcctgctggagtacggaggcaaggtt
gccattgttgctagttttgccccatcctgttaccacgcagttgtagttgtgggccagaaccacgttatcagcgggcagctgggctagtttcacatagctg
ttgagggtggcactggaggacaggtgtaggatagaaatatcatacccagcagcgatgttgttggggttccagttggcatgtttcacaatccttgatactg
agatgtactgttctgtgccatcattctggtaaatattatgatctcccaccaccacacgataagagacagccctgtccacacagtgagcagctgtcaggac
cctgttggcacggatcagggagcccccacaggtatggtaccaactgccaccagacaggtactggagagaaacctgccat
3' EST: CX981788 [+]ttgtcctttgcgggcagtgctcaaatgatatccgcttaattgaagatcatgaacgtgtaattggaggaactgaggtgcagaggaattcttggccatggca
ggtttctctccagtacctgtctggtggcagttggtaccatacctgtgggggctccctgatccgtgccaacagggtcctgacagctgctcactgtgtggac
agggctgtctcttatcgtgtggtggtgggagatcataatatttaccagaatgatggcacagaacagtacatctcagtatcaaggattgtgaaacatgcca
actggaaccccaacaacatcgctgctgggtatgatatttctatcctacacctgtcctccagtgccaccctcaacagctatgtgaaactagcccagctgcc
cgctgataacgtggttctggcccacaactacaactgcgtggtaacaggatggggcaaaactagcaacaatggcaaccttgcctccgtactccagcaggcc
cctctgcccgtgatagcccattccacctgctccagcggctcctattggggaagtacagtcaagtccacaatggtgtgtgctggtggtgatggagtccgtt
caggatgccagggtgattctggtggccctctcaactgccctgtcaacggtgtgtaccaagtccatggtgtcaccagctttgtctcctcctccggttgctc
aacatacctgaagccaactgtattcactagagtttctgcttacatcggatggatcaataataacatctaaatgactggaaagggaacacagacaagagta
agaaatgca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: