XB-CLONE-3540891
Clone Name: IMAGE:7718802
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Gene:
tra2a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981800 [+]
ccgaggctctggagttggacgcatcgggcgctgagtgtacacttgtccattccgtatcgtcattaatccgcaaaagcattcaccatgagcgacgtggagg
gaagagttaatgttcgggaagaaatggaagagttttttccaagaatattgaagataaagcaagataaaagaaggcttatgaaaagttttaaagatcagaa
aaaatcaattgaatggggaaattttttgaacagaagattgggcagatagaagcacttgaatgtttataatgctttaatgaattatttgaattggggaaga
atacacgaaggtgaaaaatgaaaaataatgaagattgaagaaaagtataaagcaagagtgaagtagaattaaaagaatctggaagaatgaatgggtccta
ggttaagtaaaatgtatggtttatgttccagtgtctgtatgataaagttaataccttgcatgatttcgtactagaacattggtcatctagttaaactagg
atttagtagggaaaatattttaatgggtttaatgcaggcattggagatttaaaaaagcaaactataacctaatcttgttttccaggtttataaatgtttc
tgtacaaagaactaggcattttggaaactgctcaagttttaatatattgtgttctactaggaatccaggtcacgttcaaagtcaccagctganagtgccc
ctcgtgtgaaatctgagagcagatccagatcccgtagtgcatcgagggcttccnaacgttcagagtctcggtcccggtcaaggtccaaatcaagatcaag
atctagaaggcattctcacagacgctattctcgctc
3' EST: CX981799 [+]aacattttaatatggtttattgtacaccgcctttactatgcacaaaaaaaaatagagtttcctttcaattaaataggctgcatgtttagcaaaagaaaag
aagtcctttcaaagatcatagtggttgggaggggaaaaaaacaaaaccctcgggaataaaaattacaaaacaatgttatacagacaccagattttcacca
gtgaatgcatagtttgtatttgcttatgatctgctaaacaaaaactacagcaaaactgtaatattcacagaacagtacatttaacaaaaatgcaaagact
aaaaaaggctaaatccaataaggttaaaaagtccaatcatgaagtcctagtagcgccttgggctgtaagaacgtgatcgtgacctggatctgtatcggct
gtaataaggtgatggggatcgtcttctgtatctgtagtcatattcatcgtatctgtcatagcctcggtcatatcccctatcataatatgagtcacggcga
cggctgcttccaccaccaccaccaccactgccgccgccactacctccaccgccgccactacctccaccaccgccgccgccaccatgggtaggtcttccca
tatatatgcctggagtgggggtatgcgctctttttgtgatagagtagtcaactcggattctcctgccatccagttccattccatttgcatgctccattgc
ctctctggaatcttcaattcgctcaaagtagacaaaggcaaagcctcttgaccttcctgtacgctgatcataaaccacattgacaccagaaaggggacca
taacgagagaacacttctctcaagtccctctctgttgtatataaacttaggccaaacactccaatacatatgttgggatctgggttggccccgactgc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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