XB-CLONE-3540864
Clone Name: IMAGE:7718852
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981818 [+]
ttcagttatacctttatttatgaagtcgctaaacttatctcggatgtctttttctaagtattccatttgatcttgcacagtcactgtgtatttcccaaat
ttcagtttcatgtcctctaccctctgcactagtgccttgttgagtgcctctccatagggagtgatgctaactttaaattctgcaatcttctgctccatct
gctggtttagctcggtgagatatggttctagtgtctgcttagcatccctagcatttacctggagtttctctttcagatcatctgcataggggctcagttg
agcttttaactgctcagtgatctgttttaattgggtgtctgcttgctcaacactctttctcatttggaattccatgctctctatctgcttggctaaattc
tcctgcatctcctctgcataagggctcaggctctcacgtaacttttgtacttgatggccaatcttatctctcacttcatcagtgtaagggttaagttgtt
ttcttagttggtctacactctcatctatttgggcctggagtttttctgaatatggtgccaaagattcttgcaaatttcctgcattgtccctaatcttaga
atccatttcttgaactacagacctaagttgctgactgattgtgttagtgttcttctctagttggcttttcagttcctcagcataaggagtcagtttgagc
tgcagctcctccaaattcttgttcacttgtttatgtacttcatctgcgtaaggagaaatcttcaccctgagctgctccagctcctgttgtatctgctctc
ga
3' EST: CX981817 [+]ttatgtgccatcacaggctcccaagccgatgtcagaacagaccaagcagtcagtgctttctgggactatgtctcccagctgcaaagcagcaccaaggata
cggtggaccagatccagcagtcagatatcaccaaacaacttaatgttctcatacaggataaaatgaaaagtatgaacctttatgctggagaactacaaag
ccaagtaattccctttgcaaagcaaatccatgaccaactaacacaagattcagagaaaattcgagagcagatacaacaggagctggagcagctcagggtg
aagatttctccttacgcagatgaagtacataaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatgctgaggaactgaaaagcc
aactagagaagaacactaacacaatcagtcagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgcaggaaatttgcaagaatc
tttggcaccatattcagaaaaactccaggcccaaatagatgagagtgtagaccaactaagaaaacaacttaacccttacactgatgaagtgagagataag
attggccatcaagtacaaaagttacgtgagagcctgagcccttatgcagaggagatgcaggagaatttagccaagcagatagagagcatggaattccaaa
tgagaaagagtgttgagcaagcagacacccaattaaaacagatcactgagcagttaanagctcaactgagcccctatgcagatgatctgaaagagaaact
ccaggtaaatgctagggatgctaagcagacactagaaccatatctcaccgagctaaaacagcagatggagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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