XB-CLONE-3540855
Clone Name: IMAGE:7718996
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Gene:
ddx21
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981824 [+]
ctaaaagaacacaacaggtgatgataatgaaccaactcctaagaaaagaaaaaaggatacaatggagatcacagcatccgaggagtgtgaagaaaagcag
ttaaccaaagaggaagaggaaataaatcaagaaaaaatagatggcgacttctctaaatttccaatttccaaggacaccattaagaatttgcaagctaaag
gcgtgtcctacctgttcccaattcaatccaaaacattccacactgcttacagtggcaaagatgtagtggtccaggcccgtacaggcacagggaaaacatt
ttcctttgctattcctttagtggagcggcttaatgaggaccaacagccgttggctagaggacgtgcgcctagggtgataattcttactcccaccagagag
ttagctattcagatcacaaatgaaatccgcagcatcactaaaaagctgaaggtctgttgtttctacggaggaactccatatcaacagcaagttttctcca
ttaaggagggatttgactttttagttggcacacctggccgtgtgagagatcttgtccaaaactaccgtttggacctcactgttttaaagcatgtggtact
agacgaagttgacatgatgtttgatatgggcttctcggaacaagtcgaggaaatattatctgttcgctacaaacctgatcctgaagaaaatccccagact
cttcttttctctgcaacatgtccagactggatgtacaatgtggctaannaatatatgaaaaaacagtttgaaaaaattgacttggttggacatagaagtc
agaaggctgccatcactngtgagcatttggccattgagtg
3' EST: CX981823 [+]tttaataagggctaagcacacacgttttatattgcataaaaatatgtctcgttactttaggaaggaatatttatgaacatggcagtatattaaaaaaagt
tattgtgacatgggatgttgggatgtttggagtcactaacggccccttctaaaccctccacttctatttctgcctcttcctcctcctcctcttcctcctc
tggaattatttcttctatcaaaaggtctcctccctcttcctccaaaacctccattccttggaccatcaaagtttctctcagattcttgaatcgcgggtaa
ttcagttgcaactgtaaactgccaccgccttgtatctttccagccctcctgcattgactgcaggttttcagaacggacatcaaaacacacacccatggaa
tccttcaggagacacattctatgaattttagaatcaatatcttcacccagttgttctttaattgatcgccatgcatagcttaagttgtgaatttgcactg
agctcttcaacgttattgtcacataacccgcttccatgttgagcaaagagcgttgtttaatggacgtcgccccagaaatatgagctaaagctgcagctag
ggcagttaatgcacccttcttctcaatcagctcctgggcgtattctttaaaatgctcaatcacatcagctggaactgaatccaaggactttattgcatca
gcactggatgatttagcaacattcaataaggaaggtattccaacacgtttgaatgtgattcccgtgctcctctccacattccgaagataatgcctttctc
ttggttcataaagggatatgcagattcctgtgcgtccggctcttcctgtgcgccctgaccgatgaacataagcatctgcttcctttg
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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