XB-CLONE-3540674
Clone Name: IMAGE:7719157
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Gene:
c9h8orf33
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981948 [+]
caatgcacaagaccattggaactgctgatattttgctgcagatatatctgttgttttatattgtgttttgctctcacactctgttccttattctcttcag
ctgcaaaatctgcacggataagtgaagtggccacaggaaccagacagaaccggagtaaagtgtttcgccagtgcctacagaactccagcagaacccagca
aatatgttctagctcttcgccttctccagagtcgcttcagaatgatttaactgctgggacatctaacggtacctccagtcaagagcggtttgttttcagg
ccgtctgaggagcctttctgctttaacttcatctaagcgtaaggtggagaatcagtgcacttcaactcctaatttattatgcagacttcaatgcccatta
taatttcattgccttcatttcattgtttagaaataagtcgctggcagcacccagtgcgcaataaaagccaaagtgccacatttttaaacccagtgggttg
gtaaaggtttcaactgtgatatatttttcttcataaatgatttgtgttaaggagtttgcaggtgatgaggttgaaaaacacttactgtacaataaacact
gctctgttacccatgttttatttctttttatatggttctgaatattcttcctatattttgatacagatacctactacctactgtgattcagtatcccctg
aagggctcataggttacattatcagactggaaaccatgccaggactgggattcagaataggccctggtatatcaagtacacagaggccaaaacagcctcc
ccaccagcccaataaatagtgactgtctatggtatcttacagcagcccctct
3' EST: CX981947 [+]cagtcgcaaggcaagaaatacccgcgtcagtggggtagggttcatttatcaagcattcatggtggtatctttcacacggatgaaatttagagttggccaa
aggattttgattggctgttgtcaactcctcagttaagcaccatatttccaaggcacggactttctcacatgctgtctgtgtcagttctccctgtcagctc
agaccactgccttccacgagagccggcctggtgctgccggcgcctgctatagtagtctggctccaccgtctcttgcacagcttgctcaatctcatccatg
atattcccaaccccaggggctggggccgatctctcctcccttatttggtaatatggataaatgaatcctggtccatggtttggcaaaaatctgaccaagg
taaaacatgttctccagaaatccagcttccaatctttgctcacattttgccacactgggaatcaaaagatggttccaggaggcctggactggcaatctgt
ggattctggcaaatgccagaggggctgctgtaagataccatagacagtcactatttattgggctggtggggaggctgttttggcctctgtgtacttgata
taccagggcctattctgaatcccagtcctggcatggtttccagtctgataatgtaacctatgagcccttcaggggatactgaatcacagtaggtagtagg
tatctgtatcaaaatataggaagaatattcagaaacatataaaaagaaataaaacatgggtaacagagcagtgtttattgtacagtaagtgttttttcac
cntcatcaccctgcaaactcctaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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