XB-CLONE-3540608
Clone Name: IMAGE:7719259
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Gene:
ddo
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX981994 [+]
agacaatgcaatgcagggttatcacatactcacacacaagcagagatagatgttatttgatagtacactctgaggaggaagagagggtgtacaccatttt
atcccacctcttagtagagtctgggcagggtaaatacacctttcagccaatacccctgttgaggtagtctgggtagatatctgatccttcaggttgataa
cccctagtatatgagtcctccgggcaagtagggatcagggttcattctatgcctgtcattatctctactccgtggccctacactgaggcaacactgcagg
ataggagaaatactcccagatctaaactctcctttgttggggctataaaactgccctttcttactaggtgctaactacatcacttacctagaaagtgcta
taacagacctactgtgcttactaaaacctcgtgcactggcctatggttctgctcccagactcaatggagcttctgcccccccccaaggaatttttcatca
ctgagcctaagcgattaagtccctatacttgtggccttgtctacacagggtctaacccttactggtctccttgttggagccctggagggtttctgcccca
gtgggccagcacaaccccagtccaacccaatattatgcacaatggaaggcaggaccaggtcggcttccagtgctagtgcttcagcgatcagcaccagggt
gccagggattaagacttacatggccatatttaacttacactggtacatggtagcatattccctcaggatgatttctttcaccattcttaaaactacagct
acaattcagctaaagaacacacatgctttgagtagctgatagcaataaaattgtacacatcctgtttgtttc
3' EST: CX981993 [+]aaaaattgctataatcggaggtggactggttggtttgtctactgcactttgcctttccgaatcactgccacaatgctcagtcactattatttctgaaacc
ttctccccaaataccactggtgatgttgcagctggatgccttattcctcatgcttatcctgacacttcacttcaacagcagaaagaatggtttaaggaga
catttgaccatttgctcaaaattgctaactcaccagaggcttcaacagctggaattagtttgctttctggatggcaaatatacaagacctctcctaagga
aatctatcctttttggtcagatctggtcctaggctttaggacaatgacagacgctgaactggcaaagtttcctggttattcttttggtcaggcttttact
accctgaagtgccagtcctctctttatctggcctggatgaagaaaaggtttcaaaaccatggtggtttggtccatagagaaaaagtaatcaatgtctggg
atcttcatggcaaatatgatgtaattgtcaactgctctggtattggatcaagaaatttgtttgatgacttatcaatatatccagtcaagggccaagtact
acaggtccatgctccttggttgaaacactttatcagagatggtgatggaagcacttatatctatcccggaataagcagcactacccttggtgggacacga
gaaaaacatgactggacactatctccagatgccaagacaagcaaagaaatctttgagaggtgttgttctcttgaaccttctcttcagggtagtagagtaa
ttga
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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