XB-CLONE-3540469
Clone Name: IMAGE:7719415
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982086 [+]
ccttatgttgttactggggaggttcatcccagacataatgaccaagaaacaatggcagcagaattataaataaaatggaacattttagggctgtaagaga
aacataaagctattcaggcacaacaaatgacagacataacacaaagacaaatctgtgttttacaaaacttgctacagtgtgcatttacttcacttttaca
ttttcttcttacttgatttgcagtaggccgacagcccaatctctgggtatttttttttctttttatgattgatatggcatacaggtggtgcaagttgtaa
tggatactgtggtctgatgacaatttgggtgtatacgttgtttagttttcagtttcagttatacctttatttatgaagtcgctaaacttatctcggatgt
ctttttctaagtattccatttgatcttgcacagtcactgtgtatttcccaaatttcagtttcatgtcctctaccctctgcactagtgccttgttgagtgc
ctctccatagggagtgatgctaactttaaattctgcaatcttctgctccatctgctggtttagctcggtgagatatggttctagtgtctgcttagcatcc
ctagcatttacctggagtttctctttcagatcatctgcataggggctcagttgagcttttaactgctcagtgatctgttttaattgngtgtctgcttgct
caacactctttctcatttggaattccatgctctctatctgcttggctaaaatctcctgcatctcctctgcataagggctcaggctctcacgtaacttttt
gtacttgatggccaatcttatctctcacttcatca
3' EST: CX982085 [+]tagggcgagaggcagatttctccttacgcagatgaagtacataaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatgctgagg
aactgaaaagccaactagagaagaacactaacacaatcagtcagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgcaggaaa
tttgcaagaatctttggcaccatattcagaaaaactccaggcccaaatagatgagagtgtagaccaactaagaaaacaacttaacccttacactgatgaa
gtgagagataagattggccatcaagtacaaaagttacgtgagagcctgagcccttatgcagaggagatgcaggagaatttagccaagcagatagagagca
tggaattccaaatgagaaagagtgttgagcaagcagacacccaattaaaacagatcactgagcagttaaaagctcaactgagcccctatgcagatgatct
gaaagagaaactccaggtaaatgctagggatgctaagcagacactagaaccatatctcaccgagctaaaccagcagatggagcagaagattgcagaattt
aaagttagcatcactccctatggagaggcactcaacaaggcactagtgcagagggtagaggacatgaaactgaaatttgggaaatacacagtgactgtgc
aagatcaaatggaatacttagaaaaagacatccgagataagtttagcgacttcataaataaaggtataactgaaactgaaaactaaacaacgtatacacc
caaattgtcatcagaccacagtatccattacaacttgcaccacctgtatgccatatncatcataaaaagaaaaaaaataccca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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