XB-CLONE-3540416
Clone Name: IMAGE:7719181
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Gene:
cog3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982121 [+]
atctgtgaactgtacatagaggaacgagctgttttacaatttgccatcaatctttcaccatgaatccatattttaaatcttccaatttgttttatatact
gagagaatgaaaccaggttcccactttaatatatatatataccataatgattataatgctgtacagggtgttaaaacgttttatgtacattaatcacttg
gtgaccttttcgcttccagaaatgcttttctggtggcaaaaggcctaaatctaatataaaagataaatatacatatgttacaaggcactaggcagttaca
gttgaagctttaaagaagagaaatctcattgtaacctgtaagctctgtactttatataaacccaacttctaaaagaactattctcccatttattccttca
attctaaacacaaggattgcatgtcaattgtgaagcataccccacacaatacctatttggtgtgtaattgcatatctatattgaaacagaatatacaggc
tgatagactgaggaaatgaatattgtccattgtgatgtccttagtatattctttaaagcaacagaataataagagaagcacaaagtgtaaatggatctaa
aagagatgcacaacacccaaaatcccattaaaccctaaactatccttaaaagaggtggaaaggcataattactggggggagggcataccccataccccac
cgcaactataatggatcacctgagaccatggactggcgctcctgtttgctggagattccgacatgttgct
3' EST: CX982120 [+]ctgactttaattagagaccttttaaaatcaagttttcagctatgtgcaagtaagtgagaatacagtaattccatgcagtaagtgacatggcatcctgttc
tgttttgcaatgctgtgaaaatgagaactacaaatcaaatttatatataaattgcaacccatgctattgagtcaggttggaagttctgaagctccattgc
acataatttattatttggacccttttttagccattttatgtctgactactgaagggtctccagaaaatgtccatattgttgctaaataaacaccaaacat
gttgggatttttttttgcttgaaatctggcattctgattatttccttagaatttccagattttccgttactttggcaactttagaatctttatattaaaa
gagaaggaaaggtacaatcactgtgggttgccaaaatgttaggaacccccctccccaccccagtgattataatcgcttacctgaccgcccaggtatgtgc
tcctgtttactgaaaactgcactgttctgggtaattctgtgtgggagcaacatgtcggaatctccagcaaacaggagcgccagtccatggtctcaggtga
tccattatagttgcggtggggtatggggtatgccctccccccagtaattatgcctttccacctcttttaaggatagtttagggtttaatgggattttggg
tgttgtgcatctcttttagatccatttacactttgtgcttctcttattattctgttgctttaaagatatactaaggacatcacaatggacaatattcatt
tcctcagtctatcagcctgtatattctgttttcatatagatatgcaatta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
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