XB-CLONE-3540379
Clone Name: IMAGE:7719089
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Gene:
XB5918646
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC157207 [+]
CTCGGGGTCTTGTTGTAGGAGAACGTTGGGGTAGATAATTACCAACAAGTTGGGTGATCCCTTCCCTTCTGCTGCAGGACCCCCATCTAATCAGTGGAGC
AGAACTGTAACTCACCACCTACTATACCTGGGTTCCATTTGGGTTTCTTGAATAGAGACAGACAGAGCGCTGACTCCTATATCCCCACAGGCCTCATTTG
CACCAGAAACAAACTATAAGGATAAGGGCCGGAAATTCTGATATCAGCATGACTTGGGCACTCGCTCTGATCCCAACCTTGGCACTGATGCACCTCTGTG
AGGGGGACAGGACTCGATTACCCAGGAGAGACGTGTTCCTCTCAAAGGAGTCCGTGTTGGATATGGCCCCTAAATCCTATGATGATCAGTACAGGGGGTG
CGCTGGGGCCATGGAGGCCAACATCCCTCAACTCTTTCAAAATGAGAAATCCTTAACCAAGACCTTTGTTGATGCCTGGGGAAAAGCCAGTAAGAGATGG
ATTTCTATGAAGGAAACCACTATGGTTCCCCCTGGATTTAGGGATGCGTACGCCATAGCAACACTGGCCTACACCAATCAGGATCCAGCCATTTACAAAC
CCTTCGGTGACGCAGTGCGCCAGGCCGGCCAGTCCAGAGACTTCTATCTCAAATACTTCCATTTCAAGGTCCTAGACTTCTGCCTGACCCGGGCCCTTCA
GATGCTGAAAGCTGTGAAACCAACAATTTGTCACAAAGTCTTCAGAGGAACGAAGGTTCGATTCAGCGCTAAGATAGGCAACGGCGTCCGGTTTGGCCAA
TTCACATCTTCATCAACAGATGACAGAGTAGCTGTGGCCTTTGGCCCTGACACCATCTTCATCATCACAACCTGCCACGGGGCCAGCATTGAGCACTTAT
CCTTCTTTCCCGATGAGAAGGAAGTGCTTATCCCTCCCTATGAGACATTCAAGGTCACCGATGTCAGATCAGGGAGTGGATATAATAATATTACACTTCA
GTCGGTGGGAACCCAAAGTAACCGCAACTGTTGGTATTTTAAAGCCATGAGCCCCGGGATGGGAAATCAGCAGATTATTCCAGTGGATTAGATTCTGCTC
CTGTGGGGCCCTGTGGGGTCTCTGCTCTTACCCCAACCCCCTGATTCCTACAGTCTGGAGAATGTCCCCATTCCCGCAAATGTAACGTAGCAGCATATGG
CGCCATCTTCTGCCCGATTTCCCTCTCATTTCTATTGTTTCTCAGCACATTGAGAATATCAACTGCATCTGCTGTGCCAAAATTGTGTTTTGTGCCTTAG
GGGGAGGGGCTTAGGGGGAGGGGCTAAGTGTATATGAAATAAGTGTATTGCATGTTTGCATTTTCATTCCATCCCGTATCACTGTTAGTGAATGAACAAT
CTCGGGGGCCCCACTGCACCCCTGATATGATGAGAGGGAGCTCATTGGTTGCTGTTGTGCCAGTGGCGCTGCCCAGATCCCATATTACATGGTGATAGCT
GGGGTGCCTCCTTGTAGGGGGGAACTCAAGCTCCTAGAGATGCACCCTAATCCTTAGGCTTTGCTGTGGCCCTTAGAATCTGGTTGAATTCTCTATGCAA
GAAACTCTGCATCACTATTTGCCAGATTTGTACCATGTGACTCATGGGGAGGGATAATATGCCCATAATACACCCTGAAACCATTCTTTGTGGCCAGTAC
TGTAGGGGGCAAATTTGAAACCTTGGTAGAACCTTGATGCCCTGTGGTTGCCCCTTACCCTCCTCTATTAAATACCCTATGGAGTAACCACAGCCTCTGA
GACATGGGCATTTCCTAGGGGGCATCTCACATTATTTAGAGGGGAGCCCTGTGGGGTGGATGAAAATACACCATCGTTGGGTCTTCTGTGCTTGGCTTTG
CTTCAATAAAGTCTGCCCTGGTTCATCCCATCAAAAAAAAAAAAAAAAAA
5' EST: CX982145 [+]gggtcttgttgtaggagaacgttggggtagataattaccaacaagttgggtgatcccttcccttctgctgcaggacccccatctaatcagtggagcagaa
ctgtaactcaccacctactatacctgggttccatttgggtttcttgaatagagacagacagagcgctgactcctatatccccacaggcctcatttgcacc
agaaacaaactataaggataagggccggaaattctgatatcagcatgacttgggcactcgctctgatcccaaccttggcactgatgcacctctgtgaggg
ggacaggactcgattacccaggagagacgtgttcctctcaaaggagtccgtgttggatatggcccctaaatcctatgatgatcagtacagggggtgcgct
ggggccatggaggccaacatccctcaactctttcaaaatgagaaatccttaaccaagacctttgttgatgcctggggaaaagccagtaagagatggattt
ctatgaaggaaaccactatggttccccctggatttagggatgcgtacgccatagcaacactggcctacaccaatcaggatccagccatttacaaaccctt
cggtgacgcagtgcgccaggccggccagtccagagacttctatctcanatacttccatttcaaggtcctagacttctgcctgacccgggcccttcagatg
ctgaaagctgtgaaaccaacaatttgtcacaaagtcttcagagggac
3' EST: CX982144 [+]gatgggatgaaccagggcagactttattgaagcaaagccaagcacagaagacccaacgatggtgtattttcatccaccccacagggctcccctctaaata
atgtgagatgccccctaggaaatgcccatgtctcagaggctgtggttactccatagggtatttaatagaggagggtaaggggcaaccacagggcatcaag
gttctaccaaggtttcaaatttgccccctacagtactggccacaaagaatggtttcagggtgtattatgggcatattatccctccccatgagtcacatgg
tacaaatctggcaaatagtgatgcagagtttcttgcatagagaattcaaccagattctaagggccacagcaaagcctaaggattagggtgcatctctagg
agcttgagttcccccctacaaggaggcaccccagctatcaccatgtaatatgggatctgggcagcgccactggcacaacagcaaccaatgagctccctct
catcatatcaggggtgcagtggggcccccgagattgttcattcactaacagtgatacgggatggaatgaaaatgcaaacatgcaatacacttatttcata
tacacttagcccctccccctaagcccctccccctaaggcacaaacacaattttggcacagcagatgcagttgatattctncatgtgctgagaaaccatag
aaatgagagggaaatcgggc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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