XB-CLONE-3540268
Clone Name: IMAGE:7719244
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Gene:
def8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982221 [+]
ccatatttacataaagacaaggatgcttcccctcaacgaaggagggtaacatttttacaggcaaatattacagataagaacgaaacttctaataagatat
ctggaattataggtctaaagactcccaagcttacagcacaggtgactgtctccctgaggttcagatttccatatttttggaagatagccacaaatacaat
atgcttcccaccaagactattgcaggcatctggatactgagactaacattcataaacttcagcagggtcagttctcagaaattcataggtagcgctgaga
gctcatgcattagatatgactgacccagtaaaatctattttcattggtaatgctaccagtgacttattagctggctaccaaggcccagtacctctcaata
acagtatctagtgttactctgggcctgtttgcacataaagcctaccattttagtttcttttacaaatagaattatgcactccgacagggatggccaaatc
aacttgtaggctaataagtcaaacattttatagctcagcccatgtgaggccagtttaagtaggggtgtattcggcagagcttcacaggcagcttttcttc
agttactgctgtcccaatcatatgggctatctgcccaaacaaatggaacaatggaaggtagccttacatgcatgtgaccactcacatgctatccatctat
ttggtctgaagactatcaccaccttaactgtgacaataatatctacatgaaatttaagttacagcagcaggagacatacaaaacaaaaataaacacatca
cctgggtacattattcaacaattctataaagagcttta
3' EST: CX982220 [+]tcttctccagctgcaggatcgccagcactttgtggaaaacgatgatatgtactcattacaagacttactagacatcagcagtgggcgcttgggttgttct
cttacagaaatccataccacatttgccaaacacataaagctggactgtgagcgttgtcaggccaagggtttcgtgtgtgaactgtgcaaggaaggagaca
tcctcttcccatttgacagtcatacatctgtgtgccaagactgtgctgctgtgttccatagggactgttattatgataactccaccagctgcccacggtg
cacgagacttaacctacggaaacaagtgcagaatcctggagaggagccatagcagaataaagtcatccagacgaacttgcattacaataggacaggattt
gagcctgtgcttgaacaatcataaattgattaaaaagcaagacaggcatgcactacatttaccactttactgactttctgtattttcttgtgcctgggag
acctacagcacattccaaaatattgcacagttaccagatcctaacctactgcaacatttggacattccagttgcattccaggaatataaggaaatggtga
ttatttctgtacagatctcctatggtgccttattagtacatggaaagcattacgtctgtatctttctgggagacctgtagcacctggattttcttgttct
ttattggttactttactcttggacctgcactcttacttgaaggtttggaggtgtatgatatatatatatatatatatatatatatgtgtgtgtgcatact
acagaatgcagagagaaactctttctatactttttaatacaacagctcagtattaatttattgactgcggccat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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