XB-CLONE-3540201
Clone Name: IMAGE:7719250
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Gene:
cel.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982267 [+]
ccctggcgatggcctgcctggtggtggcccatgccaaaacgctcggcgtagttcacaccgaagggggatttgttgaaggaaccaataagaagattggggt
tgtcttccccgattacattgatattttcaaagggatccctttcgctgctcctcccaagcccttcgaaaaagctgagaagcacccgggatggtcaggtacc
ctacaggctaaagactacaagccgcgctgtttgcaagccaccatcacccaagacaatgtcttcggaagcctagattgtctctatctgaacgtctgggtgc
ctcagacccgctctcaagtgtccacaaatctccccgttatggtatggatcttcggaggggggtttttacttggcagcggtcaaggagccaacttcctgag
caactacctgtacgacggggaagaagttgccatgcgcggcaaggtcattgtagtgaccttcaactaccgcgtgggacccttgggatttctcagcactggc
gactctaatgctcctggtaactatgggctgtgggatcagcatatggccatcgcctgggtgaagaggaacattgcagcatttggcggggacccgaacaaca
tcaccctgtttggagagtcagctggaggtgctagcgtttcactccagacactgaccccgtacaacgtgggactgattaagcgggccatcagtcagagtgg
cgtgggcctgtgcccctgggccattcagcgggaccctctcacttgggccaaatcgcttgcgtcaaagctcgggtgccctgtgaatataccaaagagctgg
cagaatgcttgcgaaacacagacccaggcgccctcactatagcctatcagctgcaactgtttaaccttg
3' EST: CX982266 [+]ctgttatgcatttggcaatttataaacgtttgtgcccagaatctgacgtagggagaacgcaagctctgcttcatggactggtagtttatcttgtggttga
tttccaggtattggccattctcagttgagtaggctagccagggggtaggaatgctggattcgcctttactggggtctccagtcgccgcaaagttggtcca
atatgcaatcatgtactgggccacatctctgtctctgggtctgtaagccagcggggtggagaacggtttgccaaacataaactgcagatcttcggcgtgg
tcagcccccatccagctggggtaaacgggcatacggcttgggtggctgaacatatagctgtatgtcttggcgcccttggcattctggtagtgtagcgcca
gagcctcctgagtagccaccaggaagatatagtctgtttccacttccacgaccgtcctcttcatgtcttcttgactgggattggtcccccagccggctgt
gtaaagatcgtacgcaatatcgagtgccggcacccctttggctagggttaagccccgcaccactttctggacctgttcggtgctgattttgtggagaggc
tggtttatagcaggcaaatcgacgccggcaaaaagatgtccatccatgttgtttacccccgctatgtagtcgacccccgctgcgttggagaataggttcc
tgggttcatcagggatgaagtctccatctatgaccggggagtaggccaagtaatggactaaaggatattcaaggttaaacagttgcagctgataggctat
agtgagggcgcctgggtctgtgtttcgcaagcaatctgccagctctttggtatattcacagggcacc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: