XB-CLONE-3540180
Clone Name: IMAGE:7719204
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Gene:
cpb1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982281 [+]
ctttcatggggaaaaagtgttccgtgtaattccacaaaatgctgaacatgttgaacttatcaaatctatggcccaaacagaagggcttgatttctggctc
cctgactcagcacaacttgtagaacaaggaaaacgagctgattttcatgctgatggtcatgtctcatatgaggttcaggcccttctccagcaaagtggga
tgccctatgaaatattgattaatgatctgcaagatgccctagaaaagcagcgcgacagcaacatcagggctgttcacagttatgagaaatacaatgatct
tgatacgattaatgcctggtctgccaacattgctgctcagaacccaggacttgtgtctcgttcctcgataggaacctcatatcaaggccgtcctatttac
ttgctcaaggttggaaaaagcggtgcaaacaagaaagctgtattcatagactgtgggtttcatgctagggaatggatctcccctgctttctgccagtggt
ttgttaaagaggctgtgagtgcatatggcgttgaatctgagtttacctctctgctcgacaacctggacatctatgttttgccagtattgaatgttgatgg
ttacgtctacacctggaccactaaccgtatgtggaggaagactcgttcagctaaccccaacagcacttgcattggaactgacccaaacagaaactttaat
gctggctggtgcactgctggagcctcaacaagagcctgtgatgaaacatactgtggctctgctcctgaatctgaaacagagacaaaggctttggctaact
tcatccgcgcaaacattccctgcat
3' EST: CX982280 [+]gtatttgtgattcatttatttgatttcattatacaccaactgttgtttctaaaataattaagaaaactttcagacctaagattaaaaatattaaatacaa
atggacatcacaagacctttagcactaagttagctttgaataacttcatttagcattgttctttatgtagctggcaatgtatttgacggccagcatggtt
tcttcacaggtaggtttaatctgagattcagggagggcaaaaccatatcgtcctgtgtcacggagctcaaatgtgtatgagaatttaacgcctgcgtcat
aggcccagtcatctgatccacctgctgcaaggtagattgttgatcctccgggaccgtaagtgtacttggttttgtacagtgaagtcagactgttaactgc
tccctgagcaactgcattcagttcattgtgatctttagcaactgcataactgtaggaataggggaacagcaacatctggctataagaatggatagtaagg
taacccttgattgcaggaatgtttgcgcggatgaagttagccaaagcctttgtctctggttcagattcaggagcagagccacagtatgtttcatcacagg
ctcttgttgaggctccagcagtgcaccagccagcattaaagtttctgtttgggtcagttccaatgcaagtgctgttggggttagctgaacgagtcttcct
ccacatacggttagtggtccaggtgtagacgtaaccatcaacattcaatactggcaaaacatagatgtccaggttgtcgagcagagaggtaaactcagat
ttcacgccatatgcactcacagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: