XB-CLONE-3540117
Clone Name: IMAGE:7719210
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Gene:
trappc11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982324 [+]
cctaatggctggatatcagcagttgccatctctgaacatcaacttactgaggttccccaatttcaccagccatctgctaaagcgcttcttaccaacacat
atatttgtaaagccacaagggcggcaagctgaagaggactccattgaagctgcctaaggaggtcctggttgttcaccagtttttagcctacatgggccgt
acagttaaactggtctggtctgatgaaggaagcatattgtaactggatccttaatatggcaaggaaaaagatacattgtggcaaggagaaagatatatta
aatgcacactaaacataaagctgtgaaagccttacctgtgtcattgacatttagcagctgttacaaaaagacattaattaaaacctaaagccactgtata
gtgcagtacagtactggaacattcaagagcacatttaaaaatgctggatcatccggaccctaaaagcatgtgagagttcccaaactctgtgatggtttct
ttattcgaatagcaggcttggtgcaagtcacatttttcttcccccccatagataaaattaaatatctcggcacctaaaacctgctgagctttatagtttt
ttttgttggagaaaagaaaggcacgatttgcacaaagcttgctatttagatggagatggttttgggatccaaatgcttgagcccgaaggcttgagtattg
taagaaacagtaacaccaaaatatgaaaatgcataacagttttaccagtgcagggcaacagtatattataatttaattactttaaaacactttgattttt
tgtggtactgttccctttatgagttgataagtaaatcagcatgcatttgg
3' EST: CX982323 [+]gagaagacagaaatgagaagttttatttggtttctttatagaaataagacaatgaatttagtaagtctactttacatgaaacaattatttacaaagcatt
attgtgacgcataacccatctttaacttaaccttatactaaatgacttctgataatgcacacagccacaataaacttaaatacacacaggttattttttt
atacaggtatgggacccattatctaaaattctcaggacctgggggtttccaggtaagggatctttccatagttggactttcatatcataagtctaaaaaa
catttaaacatgaattaaacccaataggattgctttgcctcccataattatatcttagttgggatcaagtacaaggtactgttttatttttacagagaaa
agggaataattttgtaaaaatttccttttaatgaaatctatgggagatggtctttcagtaatttggaatttctggataatgggtttttctgataatggat
cccatacctgtactacaaacccagcagtaaatgggaaaaaaaaacatccaggagctatatgatccaaattgaaacttaatcacgaatattgggaagtatt
tctcccaaatgcatgctgatttacttatcaactcattaaaggaacagtaacacaaaaaatcaaagtgttttaaagtaattaaattataatatactgttgc
cctgcactggtaaaactgttatgcattttcatattttggtgttactgtttcttacaatactcaagccttcgggctcaagcatttggatcccaaaaccatc
tccatctaaatagc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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