XB-CLONE-3540043
Clone Name: IMAGE:7719170
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Gene:
cyp3a5
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982375 [+]
actttcctcccagacttctctatggcaacgtggactctgcttgtcctgctgctgactctccttgcttattatgcaatatggccatataaattattcaaaa
gatatggtattccgggtcctacccccattccatttattggaactttccttgggaacagacatgggcttatggaatttgacatggagtgcttcaagaaata
tggaaaagtttgggggatttatgaagggcagaagcctttactcgctatagtggatcctgtgataattaaaagtatcatggtcaaggagtgttacaccaac
tttaccaacagaagggattttggtttgagcgggcccttgaagtcttctgtccttatttccaaagatgagcagtggaagaggatccgcacagtgctttcac
ctactttcacaagtggaaaattaaaacagatgtttcctctgatgaatcactatggagagttgttggtgaagaatatccacaagaaaatcaataacaagga
gccactagacatgaagcatatatttggaagttacagcatggatattgttctaagcacatcatttagcgtcaatgtggattccatgaataacccaaatgat
ccatttgtcaccaacgccaggaatctcttcacattctctttttttaatcccctgtttctgatttcaattttatgtccattccttgttcctcttttggaca
aaatgaacttctgctttctttctttgaaaatcctaaactttttcaaggatgcagttgcaagtatcaagaaaaaaaggcaaaagggcactcatgaggatag
agtggattttcttcagcttatgggtgatgcacaaagcaatgaaggcaagtcagtaccagaaga
3' EST: CX982374 [+]tttgagcatgttttcagaaatatataagcaaaaaatatgtgatccctatttacccatttcaaccattacatctaaaagaatgaatgtggttgctcctttt
attattcttccactgtctgtcctactttaggaatgagattcagaataataggttttgtgggctttaggtatcccttggtacaaattttcagtggaactgg
agtgtctttgcatgtctccaatctgaaattctgcaacaagatggtaagagcaactttcatattaactaaggcaaatctcattcctatgcagtttctggga
ccaactccaaaaggcagaaagttgtatgggtcctggttcttttgattctctttactgaatctttctggttggaattcttctggttctggccagatctcag
gattcaggtgcaatgcaaatagcggtaccacaattacaactccagctgggatagttactccattaatctccattgtcttcttacacactctgtcaattcg
tatagcaggagggaacatccttaagctctcattaatcaccatatccatatattccattttcataagtgcatcataagttggtggtgccttgttgggcagt
aaagcatcaatctcttcctcaagttttctctgtacatccgggtgcgttgctatgttatatgccaggaacatgagggttgtgcttgtagtttcatatccag
ccattatgaagataagtgactgtgcgagaatctcagtgtctgacaactctttatatccatgtttttcctcttctggtactgacttgccttcatttgcttg
tgcatcaaccataagctgaaggaaatccactctatcctcatgagtgcccttttgcctttttttcttgatacttgcaactgcatcccttgaaaagtttagg
attttc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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