XB-CLONE-3539988
Clone Name: IMAGE:7719366
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Gene:
arid4a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982413 [+]
cgaggctttggtccacccttgtctgaaggccagatgattctgtcagcttttacccattcatcatatcttacattccagccataataatgaaccaggtata
atagctctcctccatccacttccgtgctttttatatttgcctcataaattttctgagttttcccacgcccatacttcactttaactttagttccagcgag
atagtctcgatcctctgtgtcttcatcctcttcttcagtctctcccttgtgctcagaatcctcttttccttcacatggtttttcatcatcttcatcagat
tctctttctgactcatcttgctttttggttttctgatctccttttgctcggcactgtaagctccttcgcacaggagtgctttcatcttcctccttttcct
ctgttttgttcaggtcttcctcaatatccttgttttccttgttgttgtcctttctagtgtcactgtcctcaacatccgtcacaacagaaaacgcattgaa
tgcacaccttctcctcccccttggtgatttcatttcagtttcagtctcttcttttccatattcgctgctagagtccgattcatcttcctctttcattttt
gcatctaataaaggcgatgctgtctctttttgctcactgctctccattggtttctcttcttccactttaggctcttctattggtttaggatcattatggt
gaatagtcctaaactgaatatttgcagagcgacagtactcctcaaacccatacagatactttttgtatgcagtttttacattatatgacgcagctgaatt
taatatgggaataccaaggtccatataaatttgctttcacacagc
3' EST: CX982412 [+]catcctgaacattttggaactcccgtaattggtaaaaaaacaaacaggggaaggagatcgtctcttccaatgactgaagatgaaaaagaagaagaaagca
gtgaagaagaagatgaagatttaaagcgccttaatgatgaactgttgggcaaaatagtcagtgtgcaatgcagtttgcctaaggattggtttacagcact
ggttgtatctccaagctgtagtgatgatgcttctgtaaaaaaggaccagtgtttggttcgttcgtttgttgactcaaagttccattcagttgcaaggaaa
gacctcactgaagttgatgtcaatgtcctaaattcggagcattttggaaagcaaggtgtgcaggatgccattgctttcttgatctccaagacaatccctg
aaaaatggaaaatggacatgaatgaaatccttgaatcttccagcagtgatgaagaagatggagaggatgatgcttgtgacagtgatcaaaacaaagaaga
ggaggtggaaacagagcctgaagaggaggcagatccagcagagagggacatcttccttcaacaactctacaagtttatggaagacagaggtactccaatc
aataagccaccagtattaggctacaaggatctgaatctcttcaaactttttagacttgtgtaccagcaaggaggctgtgacaatattgaaagcggcgctg
tgtggaagcaaatttatatggaccttggtattcccatat
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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