XB-CLONE-3539976
Clone Name: IMAGE:7719224
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Gene:
cubn
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: EU127292 [+]
TTGGAATATCATCTGAACTGTCAACGTGACTTTATTGAGTTGCGTTTTGATAATGCTGATGGGGAACTGATTACCCGGTTATGTGGTCGCAATACTCCTT
CAGTCCCACTTGCTATTGTTGCATCTCAGGTCTGGGTTCACTTTGTGAGTAATGCAGAGGTGGAAAATATTGGCTTTAATGCCAGCTACTCCTTTACAGA
TTGTGGTGGGATACAAGCTGGAGAATATGGAGTTATTGCTAGCCCTAATTTCCCAGCTTCTTACAATAGTATGAGCCATTGTGCCTGGCTTTTAGACGCT
CCAGAAGTCCATATTATCACTCTTTCCTTTATACACTTTGATATTGAAAGCCACCAAATTTGCCGTTGGGACTCTGTTACTATACTGAATGGTGCCAGCC
CTGGATCCCCACTGATAGGACAGTTCTGTGGGACAACATCTCCTGGGACCATTCAGTCAGGATCCAACAAGCTGCTAGTGATTTTCAACTCTGATGATTC
ACTGCACGGAAGAGGATTTTATGCCAACTGGACCTCAGATTCTCTAGGCTGTGGAGGACATGTTCATGCTGATAGTGGTACCATAAGATCGCCTGCTTGG
CCTCAGCACTTTCCAGCCAATAGTCGATGTACCTGGACAATAGAGACACATGCAAGCAGCCATTATGAATTGTCATTCAATAATAACTTTCATATCCCAG
ATGATAATGGACATTGTCAAAGAAGCTATGTTAAGGTATGGAGTGGTGCTGAGGAGGAATTGCTGGCTACAGGTTGTGGGGACACTGCTCCGGCCCCTGT
CATTTCTCCAAGAAATGTGATTAGAGTAACATTCCAGTCACAAGACGCTCCAGGAAGTGGTTTCTTTGCCTCATTTATCAGCAAATGTGGTGCCAACTTC
ACAAATTCATCTGGCCGAATTATGTCTCCTAATTACCCAAATAAATATGGCAACAATTTAAGGTGCAACTATACCATTCACGCTGAAGCTAATATGTTCA
TCGTCCTGTTTTTTCAGAATTTTGAAGTTGAATCAAGCCTATCCTGTAGCAAGGACGGACTAAAAATCTTTGGTGGTGAGAGAACATCCCCCTTGGCCAA
TCTCTGTGGTGCCTCTGTCCCAGCATCTATCAGTGCAAGAGGGTCAATGTCACTCAATTTTTACACGGATTCTGATATAAACATGCATGGGTTTATGGCA
ACCTATAGAGTGATTCCCTGTGGAGGAACCTTTAATGCCTCTTATGGAACCTTAAGGAGCCCAACGTACGCCTTTACTGATTACCACAACAACATGAACT
GCACGTACCATGTCACCGTTCAGGAGAACAAGATCATAGAACTGAAATTCAATGAGCTTGATCTTGAAGCCTCTTCCTCCTGTTTATATGATTACATTGC
TGTTTATGACGGCTTAAATTCCAGCAGCCCGTTACTTGGCCGCTACTGTGGGAATGTTCTACCTCCAATTTTGAGGAGTTCGAGTAATAATTTGTTCCTG
TTGTTCGTTACGGATAATCATATAACATCACGTGGCTGGAGAGCAACTTTCAGGCAAACCTTGGGGCCTATGCAGGGCTGCGGGGGATACCTGACTAATG
CAGCCGGTACCTTTGGCTCCCCAGACTCTAATTTGGATGGAAGATATGACAGCAACTTGAACTGTGCTTGGCACATTATTGCTCCAATCAACACACAGAT
AAACCTCACCTTCAGCGCATTCTTCTTGGAGGCTCAATCTCGCAGTACATGCAGATATGATTATGTCAAGGTTTTTGATGGAAGTACCACAGATAGTAAC
CTTCAAGGAACATATTGTGGTTCCAGTAGACCAGCCCCATTCCTCTCTACAAGCAATGTTTTGACTGTTTTGTTTGTCAGTGATTCCTCAGTAGAAAGAC
AAGGTTTCAATGCATCCTATGTCACTAAAGACCTTTTATGTGGAGGAAGCTATAATGCAACATCCACTTCAGTGACCACATCATCTCCCAACTTTCCAAA
TCTTTACCCACCATTTACCTCATGTATGTGGATTATCGATGCACCAGTTAAGGAGCATGTGAAGCTGAGTGTACAAAGCTTTCGTCTGCAAACTGATGGT
GACTGCTCTCAGAATTACATAGAACTAAAGGACTCTCCTGTGGGTGATCAAGGGCAGGTCCACAGATATTGTGGAACAGAAACTTTTGGGATTCCAGATT
TTTACTCCTATGGCCAAACAGCTGTTGTGGTTTTTAAATCACAAAACTATATGCAGGGCAATGGATTCATCTTTACCTACCAGACTGCAAACTGTAGTAG
AGAATATAATCAAACATTTGGATACTTAAAAAGCCCAGGGTGGCCAGAGAATTACCCCCACAATGCTGAATGTACCACAGTCCTGAGAGCTCCACCCAAT
CACACAATCTCTCTCTTCTTTAATTCATTCAGTATAGAGGCAACAAGTTCATCCATATGTTATGACTTTTTAGAGGTGAGAAATGGAAGTGACTCCAACT
CACCGCTGATTGGAAAGTATTGTGGGGATACTCTGCCAGACCCCATTTTCTCTAAGAATAACGTCCTACGGCTGTTTTTCAAAAGTGATTTGGTATTATC
TTCTCGAGGATATGAAATCACATGGACATCGTCCCCTAACGCTTGCGGAGGTACCCTGTTTGGAGACCATGGCTCATTCACAAGCCCCAGTTATCCTGGG
AGTTACAGCAACAACACCAACTGTGAATGGACAATACTCGCCCCATTGGGAAGGCTCATTACTCTGAACTTTGCAGTACTCAACATTGATGACTCAGGAA
GCTGTGACAGTAATTATCTGAAGGTTTATAATGGGCCAGATGCCAGTTCAGCGTTAGCTGGAACATTCTGTGGAATGGGTTCCAGCATTGCTCCACTCAC
CTCCACCACTCACCAGATGTTCATTAAGTTTCATGCAGAACAAGTGGCATTGCCCTCTGGCTTCAGATTAACGTGGGTCACATGAAACCCTTTTGGGTGA
CTGGATCTGAAAAGACTTGGGTGAAATGTTATTTTCTTGCCCGACCATGAGAATATCTGTAGGAAATCCAGTAGTAGATGTGGATACTGCGTGGCCCCAG
CCTGAAAAGGATATCACATCTTGGTGCCCAATGTCAC
5' EST: CX982421 [+]gacattgggcaccaagatgtgatatccttttcaggctggggccacgcagtatccacatctactactggatttcctacagatattctcatggtcgggcaag
aaaataacatttcacccaagtcttttcagatccagtcacccaaaagggtttcatgtgacccacgttaatctgaagccagagggcaatgccacttgttctg
catgaaacttaatgaacatctggtgagtggtggaggtgagtggagcaatgctggaacccattccacagaatgttccagctaacgctgaactggcatctgg
cccattataaaccttcagataattactgtcacagcttcctgagtcatcaatgttgagtactgcaaagttcagagtaatgagccttcccaatggggcgagt
attgtccattcacagttggtgttgttgctgtaactcccaggataactggggcttgtgaatgagccatggtctccaaacagggtacctccgcaagcgttag
gggacgatgtccatgtgatttcatatcctcgagaagataataccaaatcacttttgaaaaacagccgtaggacgttattcttagagaaaatggggtctgg
cagagtatccccacaatactttccaatcagcggtgagttggagtcacttccatttctcacctctaaaaagtcataacatatggatgaacttgttgcctct
atactgaatgaattaaagaagagagagattgtgtgattgggtggagctctcaggactgtggtacattcagcattgtgggggtaattctctggccaccctg
gg
3' EST: CX982420 [+]tggaatatcatctgaactgtcaacgtgactttattgagttgcgttttgataatgctgatggggaactgattacccggttatgtggtcgcaatactccttc
agtcccacttgctattgttgcatctcaggtctgggttcactttgtgagtaatgcagaggtggaaaatattggctttaatgccagctactcctttacagat
tgtggtgggatacaagctggagaatatggagttattgctagccctaatttcccagcttcttacaatagtatgagccattgtgcctggcttttagacgctc
cagaagtccatattatcactctttcctttatacactttgatattgaaagccaccaaatttgccgttgggactctgttactatactgaatggtgccagccc
tggatccccactgataggacagttctgtgggacaacatctcctgggaccattcagtcaggatccaacaagctgctagtgattttcaactctgatgattca
ctgcacggaagaggattttatgccaactggacctcagattctctaggctgtggaggacatgttcatgctgatagtggtaccataagatcgcctgcttggc
ctcagcactttccagccaatagtcgatgtacctggacaatagagacacatgcaagcagccattatgaattgtcattcaataataactttcatatcccaga
tgataatggacattgtcaaagaagctatgttaaggtatggagtggtgctgaggaggattgctggctacaggttgtggggacactgctccggcccctgtca
tttctccaagaaatgtgattagagtaacatttcagtcacaagacgctc
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: