XB-CLONE-3539958
Clone Name: IMAGE:7719178
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Gene:
chd3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982433
[+]
ccgcattggagttatgtctttggttaaaaaaaagatacaggagtttgagcatataaatggtcgctggagcatgccggagcttatgccggaccctagccta
gactccaagctctcatccagagcctcctctcccactgtaaaaacctccaccaccactccagagccaagccttactaacaccccatgcacctccaagccag
ccacaccagccccaagtgagaggggagagaatggcgggacccctgtcgacacaggggaagcagagtgcaaggaggagaaagaaaatgatttgaagggaac
agagaagagagacattgtgcctgaacagacggcaactgcaaggactcccagtcccaaggacaccgtgaaccctgtttgtaattcagggtcggaaagaagc
agggagaagccatcggaacctcctgatctcactcctgggagggaggaaccaaaggcagaatcagcagccgctgcggagacagttggtgacaaaccatcag
ctgttgcccctgaacctgaaaaaccactagagggcatcacagagcgagacggagcattacagcggcaggaaggggttggggaaccacagagagaggaggt
gaagccagagagagaagctgtgaaggacaccaaaatgaacattcagaaggaagaagtgaagagcagcaatgggaggaaagaagagaacagaccagaaaga
ccaagatttatgtttaacattgcagatggtgggttcacagaactccataccctgtggcagaatgaggagagagcggccatctgctctggcaggctgaatg
acatctggcatcgccggcacgattactggttattggccggcattgtggtacatggctacgctcgct
3' EST: CX982432
[+]tcctatagggcgagaggcgcagaccatatgaaagacagaaccacacgaggcgttgcaggccaggcagcggtatacgggaagcgatgtgtgtgagtatata
catggtatacaacgtcggcgggaacagaggtcactggaaagggggcggggtcacgggggagactcagggggtagcactgctcggcccctttctgggaggg
gcgggaacaggattcattgcacttgtatgtcaccaagcaggagagggggccgattggtagcccccctgtgtgacactgaaagcggcagcgctatacagac
agtcccggtttgtcatcctccatcctgggtcgcccttaatactgggcttcagccattggcttagcgagtgggaggcggggtaagtgcttcagtcatcaat
gcagattacctcccctcctcggctctcctttctgtccagcaaatccatctctcgctcctttttcacaagaactgggggtccgttagaagcagacaggaaa
gagccgagaggcatctggttatagttcgtccccgtgagtgctgccgactgggggttgctggggaatgtggccccaaacatgggaggagtggtgtaagggc
caggagggaacaccggaggagctgtgctctcgctccccttgcttgcaagccggctcaggatactgcgctctgacatctgcagtcttgccgcgatgggcgg
gatacgtgcgagggaggcggggaggcgagttacatccgccttcatatcactcagcagctcctccagctggttgagcactttatgaagcacggcattggct
ggcttgttccccgctaaggactccttggagaggtgctggtgactctcagcgagacattccacctcggagaagcgagcgtta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: