XB-CLONE-3539836
Clone Name: IMAGE:7719380
|
Gene:
cela3a
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX982514 [+]
aagagatgactccgcccagattcctggaatggatctactggaatccacttacggaattctcactgatggtttgctggatccaagagttaaaggccgacac
gcgagtgaacacactgggcttcttgagggtgttgcacccaaagccagacacaaagctggccaccccatgtacgtaccaccggccatcggccccctggcag
ttgaggggtcccccagagtcaccattgcagacagagcgaatgtcccctccggcgcacaccatgctgcgtttcactgtggcgccccaccagtctctctggc
tgcagtgatcataatccaccactgggagcagcgcctgctgcaggatatcaggaatgggccccccagtgtaaagtcgcccccagccactggcatagcagga
gaaattgttggggagcagttccccggcgggggggaggcagctcagttgaaccttatcacttatctgcacagggcggggcaatcggataagggcaatgtca
ttcccacaagcgacacaaacactgttccatgattggtgcacaaacatgtcctcagatggaataaggaagatctcctctgctccttcctcattggctaagt
caaactctccaaccacgacccgattggtccgggagaagttgatacagtgggcggcagtcaggatccatctgtcggcgatgagggttcccccacaattgtg
taaaaataccccatctttcaggacctgcagagagacctgccaaggccaactgtagggtttggcgctttcaccattcaccactcgggcagatggagcgtaa
gtgggaaccccacatcca
3' EST: CX982513 [+]ctcatatagggcgagaggtggatggtggggttcccacttacgctccatctgcccgagtggtgaatggtgaaagcgccaaaccctacagttggccttggca
ggtctctctgcaggtcctgaaagatggggtatttttacacaattgtgggggaaccctcatcgccgacagatggatcctgactgccgcccactgtatcaac
ttctcccggaccaatcgggtcgtggttggagagtttgacttagccaatgaggaaggagcagaggagatcttccttattccatctgaggacatgtttgtgc
accaatcatggaacagtgtttgtgtcgcttgtgggaatgacattgcccttatccgattgccccgccctgtgcagataagtgataaggttcaactgagctg
cctcccccccgccggggaactgctccccaacaatttctcctgctatgccagtggctgggggcgactttacactggggggcccattcctgatatcctgcag
caggcgctgctcccagtggtggattatgatcactgcagccagagagactggtggggcgccacagtgaaacgcagcatggtgtgcgccggaggggacattc
gctctgtctgcaatggtgactctgggggacccctcaactgccagggggccgatggccggtggtacgtacatggggtggccagctttgtgtctggctttgg
gtgcaacaccctcaagaagcccagtgtgttcactcgcgtgtcggcctttaactcttggatccagcaaaccatcagtgagaattccgtaagtggattccag
tagatccattccaggaatct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: