XB-CLONE-3539819
Clone Name: IMAGE:7719334
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Gene:
etnppl
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982526 [+]
tgataggagacatcagaggagttggcttgtttgttggagtagatttggttaaggacagactgcttagaactcctgcaactgctgaggcccagcatataat
atataaattaaaagaaaagagaatcctactgagtgctgatggcccttacagaaatgttctgaaattcaagcctccaatgtgctttaccaaggaggatgct
aaactggttgtagatgagattgataaatgccttacagttcttgaaaaggcaatagaaggccaatcaaattctggtatacatgaaaaaaccgcagccaaga
gaaaggtgcataatgaaaattcaggagacactaacacaaaagaagaggaaacatgtaccagcagcttacgagaaagcaacctaaatgacaatgcctacag
acaaagcaacggactgcatcctgggagccccaaagtcacacgtaaaagaataagaacataaaggaggtttctgagttcagaatcttcaaactgttgctgc
cttcctattctaacaactctaacaaatgaagatgcttggtctagtctggcgaagggttgttaaatttgatttgccaatgaattttaggggtttatgtggt
gcatcctttttatttttttttattctgtatttgcagtttcagtatatcatattggaatggaatcttttcccatgttttaatggcttagatttaagatgca
gtttaaaagacctcagcatgctgaatgtactgagcatgctatgtacaagaaaattttaagccgtttccaactgtttagatacaccaagattttagctctt
gctcaaataagtaacccttttccactcgctacattattttacttttttataggggtccctacagatctgatgtagcagcctgaaatccctgtgtacatg
3' EST: CX982525 [+]aatgaataagcttttatttcttattatgttttgaatctagtttatagagaacaaatgatatactgtagatatgcatggcatggatttagccaagcagtag
gatctggtataatgtgattatggatatctgctcttctggcactctttcatagtcattcttgtcaagtgctgttatacatctgaaatgttctgacacttta
agaaaagaagcatacgcatgtttacctagttttaagaaaactgctgatcccccatgtagcttctcattttaaaatataaaacattatagcaccttgtatg
ttgacaaaaaagaaaataccataggcagtgagaaaatgcccccttatgctattctatggggctcatattcaagtaaaagggatataggaatcatgtacac
agggatttcaggctgctacatcagatctgtagggacccctaataaaaagtaaaataatgtagcgagtggaaaagggttacttatttgagcaagagctaaa
atcttggtgtatctaaacagttggaaacggcttaaaattttcttgtacatagcatgctcagtacattcagcatgctgaggtcttttaaactgcatcttaa
atctaagccattaaaacatgggaaaagattccattccaatatgatatactgaaactgcaaatacagaataaaaaaaaataaaaaggatgcaccacataaa
cccctaaaattcattggcaaatcaaatttaacaacccttcgccagactagaccaagcatcttcatttgtt
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
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