XB-CLONE-3539816
Clone Name: IMAGE:7719382
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Gene:
sgk2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982528 [+]
caaatacaatttattatctgtatcacatttttataaaaatatagtccttggtttcaggcacaatatgcgcagaccttgcctccaaaaatgaaacggaatg
ttgaaccaggaggaaaatatttatttcgtgaaaatcgacgtttttgtgttgcagctgagcgggtcgtgatgggataaatgaagtgttttggtagaaaaag
gacagaactgagtaagtgatacagtgggttatgggtaacgtcgctgagtttgcctaaggatccagttaggcagatatcatagagctgaatgccccctgca
taccatggttgctattgtaaggggcaactccggcttcagaacctaaattagtggggaggaacagggggcggggcttagggaactgttccaaaccatatta
ttacattaaaaatcattaataggctgcatttttttaaactgatgtatattgcaaagttgtttaaaattatgtttacttttcttaaagcttaagttgtttt
taggtggagttaagtaggctatcagtaactggctgctgtgaactgaatcacaagcagccaatagcttatcttattatgtgcctcttgggcctataatctg
aaaagagcagtgtcggactggcccatcaggatacccagaaaactcctagtgggcccaggtgtcagtgggcccttctgcctgatcatttgtcctgcttcgt
ggtcattccctatttctgaatcggaacaagtagagaaatagatggagaagtaaattctagcatgtaaaagagactagggggcagattttatcatacanca
gttcgaatcccgaatgggaaaaactc
3' EST: CX982527 [+]ggagaggttccctcttctctgctttcccctcgggcacaaactacctcttgagccagtactggttttccttctttgtgatagctttctagttcctgttcct
aacccttcctcatactgtcccttcttccaccctctctttctttttgtcccctcgctcttcttttctcagagtttctacctttctttgttttgtacctaac
tagtttggggaaccaggtaataatataaatgtctatcctcctgctgtctggttgactaatcctggcaaccaggctaaccagagaatagaaaggcaaatca
tttaaaaaaacaaagaacaagtaactgtggcttagatgaatccatgtagaacagtacattgcaagctcacattgaccacatttacacataaatctggcag
gcagcattttcgcagcaaaactcaaaatggcattttactctttatacagtgtcagacttggacctcagggcccaccagaaaaccataggcccactatcca
aactatttttcctccttttctcgcccgaccacttcatactcctaggggcagatttatcaatacacgaacgctgggagcgttcatccgaacgctccgagcg
catcttcaccgattttttcgggccccgcacgacaatttcggacgccctcgcgatgctttcacaaaggattcggagaggctctgccgctgtttgcagttgt
tcggtgcgaaagtttcggcactttcggatccccaatgcgatattgtcgtgactggtacgattttttcgtgggcattttcgtgatgtttgcgatctttaga
ggtttccgagttttttcca
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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