XB-CLONE-3539749
Clone Name: IMAGE:7719437
|
Gene:
stra6l
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC160523 [+]
attctgaacacatcataaagactgaacagaaatttcccttgtttctttccatagctgactcaatctaggattcaaatgtcctccgacgaaaacatcgtgg
acttccaggaggacaaaatggaaaattccacctgcacaaaatatgtagatatggaattgttcctgcattgctcacttgttccctccattcttatcatttt
ggcactttctttccttgaaaaacgtgttaacaagtcaaaactagacaacaaattttaccttctcagtggaagatttggaatagtcattcctttagatctt
attggaactttcagcaacaggtggacctatggttttgcatttggtgccatagccaaccaagtcctatccctgttttcagaggaatatcttcccataggag
tgcctacctgggcaaaagcccttagtcttttcattggagccattgaggttggactgtcattttatcctatttttgcctgtctcagcactgcaaataaatt
aattgggtctattactggatttctatacactctcacttggtttatcatcgcaaccatagacattgtctcgtgcccagatggaaatgttataggccgctat
gaaaatatcatcttttattggccctgcctgctctgctttatatttctgcttatcaggtttgtttacctgttcataaagtccatcagagtgattataggtg
tggaggatgtcattgatgaaggaaattgttttttgccaatacatcaagctgaacatgtgaaaagactttttagaaaacctgtggtggagaaaaagtcctg
gtttgcaagaaaaatatatgattctgatccttgttttaaattccccagcagaatgattggcactacagtcctgtccttgctgtgtctgtacatttttgtt
actatagaatttaaattcattattgctcttttacgattgctgacaaattttgaaggattactagaagatcctaaatccgatttttacgtatttaatgaat
cgatcacaactgtaaaggaattccacagtgctttggagggtgtatggtttttcagtatcttcttagcaagtgccataacagtttcctacatatttcacat
tttagtctgctacagaaaacacatgaagcggctttggataggaaacaggtcattcctgcctgtaaagtttcaagttccctctccctatgagagtgtggca
gcgattgcaagatattctggatggcaagttgcataccttttgtggggttacatcataatgcatattgttcagataatattaggattggcttttgcgtatg
cttttgtttatccaataaagcacggtgaagcacttaagttgtttaaaggcttgggtatcttcattctcacgattggcatcgtgattgctcttatgatgct
gcaagttgtactcgctcgcctgttctttctgcagcagaaaatctcttgtaatgataagcagaaacctttggcactcaacaacaggaaggcatttcacaac
ttcaattatttctttttcttctacaatgtgattgttggactgagcgcgtgcctattgagattgctcagaagtgtgatactggggagctggctaatagctc
ggattgacaggtcaatactgcaaaggggatttgaatcaggtgacacaggctacacgacatggattgggatgatatatgtggatcattatcacacaaaccc
agtgcttgtgagcttttgcaacattcttttaacaaagagggccgagaaaacagtaagagaatgctcttcataccatggattctctgactcaggttcaaat
attcaagcccggacacgatggcttctatattacactctcttgaaaaacccaagacttatattgcaaaggaaaatgaaagaactgggcgatgcagaattga
gcaaagccataacccacgctagaactgttcaggctactgttatggaggctggagtaagaaatatcttcgaattaccaattgatgtaaactgcgtggagat
tagagaacggcgatcatttgactctaagcaggttacaggaaatgattcagaatattaatttaagactgaacaaggttttctaaaggtcaaaaaatacctt
tttttagaactgaaatgtttaatttattatgtgtggtctagtttccccttcaaatatttaccctatactaaaacaaagaaggcaagactgtagaaaattg
cttctgttgcttactactactgtgttgtatgctgtataacaaatgcagtaaaatacttttaaccaaa
5' EST: CX982573 [+]attctgaacacatcataaagactgaacagaaatttcccttgtttctttccatagctgactcaatctaggattcaaatgtcctccgacgaaaacatcgtgg
acttccaggaggacaaaatggaaaattccacctgcacaaaatatgtagatatggaattgttcctgcattgctcacttgttccctccattcttatcatttt
ggcactttctttccttgaaaaacgtgttaacaagtcaaaactagacaacaaattttaccttctcagtggaagatttggaatagtcattcctttagatctt
attggaactttcagcaacaggtggacctatggttttgcatttggtgccatagccaaccaagtcctatccctgttttcagaggaatatcttcccataggag
tgcctacctgggcaaaagcccttagtcttttcattggagccattgaggttggactgtcattttatcctatttttgcctgtctcagcactgcaaataaatt
aattgggtctattactggatttctatacactctcacttggtttatcatcgcaaccatagacattgtctcgtgcccagatggaaatgttataggccgctat
gaaaatatcatcttttattggccctgcctgctctgctttatatttctgcttatcaggtttgtttacctgttcataaagtccatcagagtgattataggtg
tggaggatgtcattgatgaaggaaattgttttttgccaatacatcaagctgaacatgtgaaaagactttttagaaaacctgtggtggagaaaaagtcctg
gtttgccagaaaaatatatgattctgatccttgttttaaattccccagcagaatgattggcactacagtcctgtccttgctgtgtctgtacatttttgtt
actatagaatttaa
3' EST: CX982572 [+]tttggttaaagtattttactgcatttgttatacagcatacaacacagtagtagtaagcaacagaagcaattttctacagtcttgccttctttgttttagt
atagggtaaatatttgaaggggaaactagaccacacataataaattaaacatttcagttctaaaaaaaggtattttttgacctttagaaaaccttgttca
gtcttaaattaatattctgaatcatttcctgtaacctgcttagagtcaaatgatcgccgttctctaatctccacgcagtttacatcaattggtaattcga
agatatttcttactccagcctccataacagtagcctgaacagttctagcgtgggttatggctttgctcaattctgcatcgcccagttctttcattttcct
ttgcaatataagtcttgggtttttcaagagagtgtaatatagaagccatcgtgtccgggcttgaatatttgaacctgagtcagagaatccatggtatgaa
gagcattctcttactgttttctcggccctctttgttaaaagaatgttgcaaaagctcacaagcactgggtttgtgtgataatgatccacatatatcatcc
caatccatgtcgtgtagcctgtgtcacctgattcaaatcccctttgcagtattgacctgtcaatccgagctattagccagctccccagtatcacacttct
gagcaatctcaataggcacgcgctcagtccaacaatcacattgtagaagaaaaagaaataattgaagttgtgaaatgccttcctgttgttgagtgccaaa
ggtttctgcttatcattacaagagattttctgctgcagaaagaacaggcgagcgagtacaacttgcagcatcataagagcaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: