XB-CLONE-3539686
Clone Name: IMAGE:7719777
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Gene:
clrn3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982616 [+]
ctaaggtgcctatcacattcacacatactagaatagagtcctgtagcagctcaggggcctgtgcatttaaagaaggtaccttgtgggttgctggtagggt
ttctggatgcgagtatagatgcgggttttgggtctgttggtcttatctgtatttcttattttacattatagtatctatcttatctatattttaccccata
aacattttttaaatgtttttttttacccccgcccactgctgatgatgtcacttctggtttgcagcaactgcactatatatttaattgtaattagagggtt
gcggatcgggttgcagacaaggcagttgcgggttggctagtgggtcccaacaggtagaaatgcaggttgcagggccagattatgggttgcaggctgtggg
acctggtcgggttcgggttttacgctacacttgggtcagttttatcccctgcctgtatgtacagtatcagcaccccaagcactacagaaacttgtaaaca
tggaaaatcttccaaaggttgtgaaaatagatgttaatatttctggaagaggagagaaagcaatgttcttattggctaaggggaccttccacagagagcc
tcctgttctgttggggagcaaaaagaaagttaaataatcagtcacttgcactctgtgaaactgctgtccgtcctgccttcaaaagagtataacatcttgg
gtcgccttttccatcggcctctcttgttccttttgcttagtgtattttgcttgctgataaacataaataactgctacggtggcgaaattgagagctatta
tcaggattaatagccagaaggaatatccgtaggagacgcgcgttttgtaaaactgatcttcagctttatccagcgtcatggctag
3' EST: CX982615 [+]aggaatatgccatccaagcagaagacattattatttttgtccggttttgtagccagtattggatcttttgccattgtttgtacctgcttagcaacaacgg
aatgggtatcgagtaaggttgattacaggaaagataattccagcggttatgtgaatattcagtatggactcttccaaggaacccatgccaagattcctct
aacagcaggacttggctcagaaacaatcaaatttcaagttatggattatgtaaaagacagcgctaaaataatccagatagtgatcatcttgttccttgtc
ctgggcctgatctgttcgttcctaagttctgcaatcacttgcctgaacagtgtgagcaacccttacctgacttacctgggacccattggagtctatgtct
gtgtgtcattaaatgccatcctcagcctgctctgcatgattctgtgcgctgccaatgcagagataaccgggatgccaaagcaactagccatgacgctgga
taaagctggagatcagttttacaaaacgcgcgtctcctacggatattccttctggctattaatcctgataatagctctcaatttcgccaccgtagcagtt
atttatgtttatcagcaagcaaaatacactaagcaaaaggaacaagagaggccgatggaaaaggcgacccaagatgttatactcttttgaaggcaggacg
gacagcagtttcacagagtgcaagtgactgattatttaactttctttttgctccccaacagaacaggaggctctctgtggaaggtccccttagccaataa
gaacattgctttctctcctcttccagaaatattaacatcta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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