XB-CLONE-3539639
Clone Name: IMAGE:7719781
|
Gene:
prag1
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CX982647 [+]
atcctgcaagaactngctttcacccacgaagtgaccacagacagttacaaccactctctgatgggaaaaacggaaacctaccagcattgcatagccttaa
tgggattaaacttaaacctgacatcagtcaggatgaagatactgtaatagcgtctttgtataccaaacccacgattgctgtgaaaccaaccatgcttacc
tcagataatgagatgtggacagagattaacagcaccgagaccctaactcaggttagttggaagatgtctcagacaagcaattctcttctgaaatctgggg
atatttctaagtcaatccttgataattttaacaagcccttcaatcagaagttcagcgctttcaatgacaatactcccagctatgtttttaatggactaaa
gagccctgacagaaagatggggataaatgctgtgaataacctaacctatgtgagccactttagtggtcagtctgataaagcaacctttattaaggagaaa
ttgcccattacctttgaacaaactactaatggaaacctagttaatggagacataaagaaattaagctgcattcaggaagaatctacgttactttctttaa
aaagcagtagtggttgctcctctggaactctatcctctacagacaggaacactccacctggaactttacttacaagaggcacttcagtacctcagggaac
cggtaaatctggaatgggactgtgtaatttggataacccaacacctataaagcctgctagaaaatttcaagataaaaactgcttaaaaagcttagagctg
ggatctagtaagaacctgaattgcagcagtcctaaagttacacttcattctgagtcagatgccctgacaaacaatttgtcacatacccttttac
3' EST: CX982646 [+]caaagttcagtatacagaagtacaaaaaagacaaaggagtagtttcctaactttcttgtatatcatgttaagtacttacatttgaaattgttttgtaaac
attttaaaccttaattgcccaagtgaccaatatatatcattgctctgcaatgtgtttaaggcccttagacagaacgcaatatgacaatttgatatataca
agcatatacacatctacaatgtacaaaaatatatttacacatttttttatactacggatctaaaaatcagtggctctccagctgctgtctaactacaact
cccagcgctctctgaaaacatatcggatgctgggaggatgtacataagctacagtttaatggcaactttactgatttaaagcttaagacagttgaatcta
ataatcaagctgtgtaggtaactctcatggttatgaaaatgtatatttatattttgtgtgtgtttataaagctagtgggggaggggggcattttgtaact
ctcaatgttgctgaacacaaaactactttactactacacataccctgtgtattgaaatgaatacctccactaacttaaccgttagtcttaatgaggataa
aaactacagccaccagctcctgtattttctgaaagcaaagaaaatgcagctcctgtaacatacagttgggtgactgaaaataacagtgctgttgtctagt
aaatgcactagaaaacaatttctgttaatagtgacaaaactttcaacatctcaacatatttttaaaatattactctcttttcaaaccagtatgaggagtt
acaagaaaagcctgccaacaggtttataagacgtacatgtcatttaatttatactggagcagctctgaacactaaaagttgcagtgaccctatttgacaa
ctagattta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
Suppliers: