XB-CLONE-3539587
Clone Name: IMAGE:7719547
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Gene:
apoa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982684 [+]
tgcattttattgagaagcattgataaagtgcatgcatatcaaccacaacgtaaaacattacagggccttatgttgttactggggaggttcatcccagaca
taatgaccaagaaacaatggcagcagaattataaataaaatggaacattttagggctgtaagagaaacataaagctattcaggcacaacaaatgacagac
ataacacaaagacaaatctgtgttttacaaaacttgctacagtgtgcatttacttcacttttacattttcttcttacttgatttgcagtaggccgacagc
ccaatctctgggtatttttttttctttttatgattgatatggcatacaggtggtgcaagttgtaatggatactgtggtctgatgacaatttgggtgtata
cgttgtttagttttcagtttcagttatacctttatttatgaagtcgctaaacttatctcggatgtctttttctaagtattccatttgatcttgcacagtc
actgtgtatttcccaaatttcagtttcatgtcctctaccctctgcactagtgccttgttgagtgcctctccatagggagtgatgctaactttaaattctg
caatcttctgctccatctgctggtttagctcggtgagatatggttctagtgtctgcttagcatccctagcatttacctggagtttctctttcagatcatc
tgcataggggctcagttgagcttttaactgctcagtgatctgttttaattgggtgtctgcttgctcaacactctttctcatttggaattccatgctctct
atctgcttggctaaattctcctgcatctcctctgcataagggctcaggctctcacgtaacttttgtacttgatggccaatcttatctctcacttcatcag
tgtaag
3' EST: CX982683 [+]ggttttatgtgccatcacaggctcccaagccgatgtcagaacagaccaagcagtcagtgctttctgggactatgtctcccagctgcaaagcagcaccaag
gatacggtggaccagatccagcagtcagatatcaccaaacaacttaatgttctcatacaggataaaatgaaaagtatgaacctttatgctggagaactac
aaagccaagtaattccctttgcaaagcaaatccatgaccaactaacacaagattcagagaaaattcgagagcagatacaacaggagctggagcagctcag
ggtgaagatttctccttacgcagatgaagtacataaacaagtgaacaagaatttggaggagctgcagctcaaactgactccttatgctgaggaactgaaa
agccaactagagaagaacactaacacaatcagtcagcaacttaggtctgtagttcaagaaatggattctaagattagggacaatgcaggaaatttgcaag
aatctttggcaccatattcagaaaaactccaggcccaaatagatgagagtgtagaccaactaagaaaacaacttaacccttacactgatgaagtgagaga
taagattggccatcaagtacaaaagttacgtgagagcctgagcccttatgcagaggagatgcaggagaatttagccaagcagatagagagcatggaattc
caaatgagaaagagtgttgagcaagcagacacccaattaaaacagatcactgagcagttaaaagctcaactgagcccctatgcagatgatctgaaagaga
aactccaggtaaatgctagggatgctaagcagacactagaaccatatctcaccgagctaaaccagcagatggagcagaagattgcagaatttaaagttag
catcactccctatggag
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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