XB-CLONE-3539563
Clone Name: IMAGE:7719597
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Gene:
c4bpa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982700 [+]
cataaatagcactcacggcttatgaatcaataaaaaacctttattagttttccatatcgacgcgtttcgacccactagggatcgtcatcaggatatatat
atatatatatatatatatatatatatatatatatatatatatatatatatatatagtataaaatattgttaaaaagaaactattataataatttccaagg
accacagtgcactgtgctcacatgcaaaacaagggtatgcatgcatgctaggatacatcagccaataaacaaacagctgcattatttcctgtcaggtgaa
ctctgagggagcacacagaccatcatagagtggtggctcaaggtaaactatgtaaaagaggagtatttaccaacatatttactaatttggtaagattttt
ttattaggttacttatgaggatatacattatctgttggttaagtattcattctgtgggtaaagctaaccaacagataattgatatcataagtaatcctga
taacaattttataaaatgtttatatattacactttttacaacttttttctcaagccactgttttgtgatttctgtttgcgtctcactgatcacctagtag
gaaataatgcaggatctaactgtaacaggaataagtgtaggactgaaagctctatgatatagccatttattggctgtaacctgatgtgaagcacagtgcc
acataaaagccagagggtggactactactagggggccatttactaatgctcacatttttgggggatttttagtgctagaagtggcagaaaaaaaaagttg
caacttttcaatatttactatgcatccaaacagctaanattctagtccaacaattcaccagctaaaacttgctg
3' EST: CX982699 [+]tttttttaaggtagaatgattttattaatgttcaacccaattaaataaataaagagcagcaaatgcatttgtaaaacaaaattctgatacctgccaaccc
ctttgccctcccacctaagaatataatatcattgtacctaatatcagaagtgtacaagttttggacaaaaagggaaactcgtgggcttacttagcttgtg
atacaaacaaaataacttgcgtggtgtcaaagagtgagtgaaggtgacaagttaaaattaaggaacagattaggtagggttaacaggcaccgatatgtta
aaaaaaaaaattctctttggcctcttttacattacagtgccttgtgtgtaaggtatctctttgggattatattagacaaccttaatcacatccagttaca
gtctatggaaggcagtcagacggaacaaactagcagttgctatggttgagcacattttgcaatgagcttttttatgtccaatatcaatacatttgacttc
aatcacacaatagaagtgtaaaataaatcttgggataaagtacattaataatagagcatcattacaaatacagtgtgtagtgtactatggagataaataa
aataagacccagagctaacaactacttctcctgactcaagcctagcagttgttagcactttttatatgttatgctttacttatacagtattgacatatta
cttgtaagcattcacatcattccctgagtcagtgtttctaaaggcaattaacccattgcagcaagaaaaaaaaaacccttataacagggatactatgcac
aatctctgcaggggtaccacagacccaagcccaaaatcaagggccttttggtatttaacatcccacgaaaaggatttggcaaggtgtct
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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