XB-CLONE-3539527
Clone Name: IMAGE:7719457
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Gene:
hla-dra
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mhc2-daa
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982724 [+]
caaacatacagtatttaatgcataaggcagaaggcacaaaactaaagaaaatgtcctacgtgagatttaaacagcactctcttataagatataaatctct
taagatatagttctgtcttcaatcatgtgttgcattccagctaccgcagggttacaagcattattactatactgatcatctctacagaaagacaaaagtt
aaatgaattatagggttggggttacactttttaatagctttatttttgtttaggagatcaagaaaaacagcagggcatccatttagtttgtacagaaagt
agcaatcaagatcagggctatataagaaaatacaagaaagtacatgactggatagaatactttgcagttctggagctaccagaccatagagcaagccaat
taacagaggatgttacaacttcaacattcaataacgatcctccttgtagctgggggtaatttgatattcattcaaagccaacacatacattgtattttaa
atcatcatcaaataaaattgtgggttagaatgatatacatcacacagaaaccattgcaaatattgaggctgcaattaaatgtaattttcaatttcagaga
tgcatctaaaagctaagctaatttaaaaacaaaaaaatcaaggaatcaatctttcatacactgtagcagagattatagtatgtgtgatgcccaatgatat
atgagtgctatttcatggtgaatatgaacagtttctattctgtttgtgtaagcaaggtatattcagcanaacaaaggagaacagatacaccacattaaaa
gaatacaaaattgtcccactgatttgcataccacccctcagcactagtataagcac
3' EST: CX982723 [+]gcccagtgacgctgtaacagttgattacttcgattattctgcgttattctatcagacccgcagacccacaggggaatatctgtttgactataacgggaat
gagttgttccacgtggatttggactcgaagtctgtggtttggacgctccccgagccgactgccccattgcccgtaggtgcggatccggtggatctgcccg
tgtatggccagctttagtctgtaatgcaggcaatgtaacttgaggctcaaagcagagaatgttgtaatgcttatagctctgatatttttatgaatcatac
ttcactgaaataatattgtaaatttcccctcccaatgcaatatcattttcttcaaattggaaatccaatgtatttacctgttcatttaaccttaattatc
ccatcccaaaccattcttgtacttaattctgtttagagaagggcatactgatagccagcaaagatcatcagcactgggttatctgaaaggcactcacata
atgccagaagctgcagcattacccagacatttttccactgacactcagcaatttgactgcagtaggaagtttctggagacttgaattaaactcaaagaaa
agtctctggtctcattctgaaataactgtatctatattctgcatggctggaggcatttttttcttttatttcttaaagggataagtttaacaaatgctta
ttttcagtacaactgtacaataacaatgactatttcagttccttcctactagatcctattaaatcccatagatttaagttaaacttcctccctctggtgt
ttgtccactacactataaacagacagtctgaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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