XB-CLONE-3539471
Clone Name: IMAGE:7719605
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Gene:
sypl1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982761
[+]
attgcatcagcaggtacagaatagtttttttttatctgattcagaacaataattcatatacatggagaaaaacagttaggcatataaacagtattgggaa
atagggtaaatgtgtgtctagaatgcaaacttgtggcaaaagctacaatagggctgtaattgcttgtacatattcagtaacctgcacaaccatctatttc
cattaacattttcaacagtgctctaaaaaatagtacatgtaaaagagacgaacttggggttttgtgcaataaaataagagaaacaaatgttcaaaatatc
tagaatataaaaaaaagcatttatactggtaagcttttatattaaacttaagcattgcagagagtttgcctattatatgacaaaagcttttggtttaaga
ggaaatcccaatttttatagttacatcagaataggtattccaaattagtacagtacttcatattctgtcatttttcaaaacaacatattgaagaaatatt
tatcagtgtttgcactcctaaaaaccttagtgttttaaagccaataaactgcagtacatcactgttcacaacaaaacattgttctgtaactggaaaacct
gcttgaaataagtcgtaggcctgtttttcagaaagaggaatcctggcatgaataccttgtatccacatgtttattaacacataaacaatgcaatccttgg
cattgaaaacaccagattgtaggccattaaatgtgaattggcatttctataggatttatctttaaattcatggagaaacatttccaggtgcttgctccgg
gtttggttgttgtggaggagtatgtaggcttgtctccttgtatacaaaccaagcatttcctgcccacagtatcagattcaaaaatccaaa
3' EST: CX982760
[+]cggctggcagctgaaccttggcccgctaaaggagccctctgggctttatcaaattattggaatggtttgcttcaatcttttcatttgccacttgtggtgg
ttacagtggcaaaactgaattaatagtaagctgtttaggatcttcagaaaataaaactgctaccgcttcatttatgtatccgtttcgattaaatgttcaa
gtgtttgaagggatacccaaattattctgtaatcaaacatggaaagactttcaccttacaggagatttttctgccccagcccagttctacgtttctattg
gagtctttgcctttctttactgcatggctgctcttgtgttttatctaggttttatgcagttgtatcgggactctcaaaaattcccattatgtgacttctt
cattacggtcttctttgctttaatgtggctggttagttcctcagcttgggctaaaggtttgacagatataaaagcctccaccagccctcaaggtattgca
cagtatacctgcccgactggttacaaatgtctgcctgggcaagagtcacacatgggaagtctgaacatctctgtggcgtttggatttttgaatctgatac
tgtgggcaggaaatgcttggtttgtatacaaggagacaagcctacatactcctccacaacaaccaaacccggagcaagcacctggaaatgtttctccatg
aatttaaagataaatcctatagaaatgccaattcacatttaatggcctacaatctggtgttttcaatgccaaggattgcattgtttatgtgttaataaac
atgtggatacaaggtattcatgccaggattcctctttctgaaaacaggcctacgacttatttcagcaggttttccagtacagaacaatgttttgttgtga
acagtgatgtactgcagtttattggcttaaa
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-40407.jpg) |
Usage:
Suppliers: