XB-CLONE-3539468
Clone Name: IMAGE:7719653
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Gene:
dpp9
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982763 [+]
tgtgaatggtgcaaatatgtgcacacaagtacacagagaggctgaggcagtaactggctgaagacctgaagtgctctgttatagaggtcacattctgcaa
atcagtgttaaacgcgatacagatgggaaaagcgttatgagtaaaactagaggaggagcatgttggctggcatttattatatcgtgcgcacagagctcac
aagtgcagcaagcctatgtatgtaagttagataagaaggaaaggaaacatggtcatcttaaagtaattcccaaacagaaagaaagtcattattccaatta
atttagatgctgaattgaagacttcatcatggtgatcagtgttatgcaataatccatctcacaccaatcactgttcagcagtaccttgttgcaatgctag
taataaaacacattccaaaagattaattcttttgaacttttctccaaaaaaaaaaaaaaagtttccttctcttatagcttccatttctgcttttttccat
ttaaggaattgtctgcttgtttccttcactaatcctgttcccttataaatgttcttgcaaaaagtgcagtaaggtgatttcataatgttctccagactca
gggcatcggatactgtgcctttcattggggtaaatctgaagttgataaggttttcctgctcgaatcagttgagacaataagaaattagtatgaaaaaagt
gcacattctcatctagaaagccatgaaggattagcagtcgatggggctcatttggaagtttgtccacatgaagtgccgcagatccagcttcatatccaac
ttggttgtttactggagtatccatgtagcgttctgtatatccagtgtcatatgccatccata
3' EST: CX982762 [+]agtttgaccgcttcactggatactggtggagtccaactgcagtagacctcccaggagggttttctgtgcttcgtatcctgtatgaagaggtagatgagtc
agatgtggaaataatacatgttccatcaccggcactggaagagaggaagactgatgcatacagataccctcgcacgggtgaggtggggtggaccgctcac
atgagatgagtagggacggctaacgtcgctattggaagcaagaatcccaaaattactctgaaactatcagaaataacaaccaataatgatggtgagattg
ttgctgctcaggataaggaattggttcttccattccattcactcttcccttcagcagaatatattgtaagagctggctgggcaaaggatggaaaatttgc
ttgggctatgcttctggatcgtccccagtgcctcttgcagttagttttgcttccacctgaactatttatcccggtgtgtgaagatgaagagcaacgaaaa
caatatgtaaaggaagtgcctccgcatatccagccatatgtaatatatgaagaaaagagtgacacatgggtcaatgttcatgatgttttccacctcctaa
gacaggaaaaggaggatgaaattagtttcatttgcagcagtgagagtcagactgaattctgccatctctatctgatgacatctttgttgcaaaacgagca
ccgagaatggtcacaaatgatcagcccatgcacagtgganatgtttaaatgccatataaagcaggaggtggcattaactagtggagactgggaagtactg
tgtcgtcatggagctaaggtctgggtaaatgaggcaacacagctagtttattttcaaggcacanaggacactcccttggagcatcatctatatgtta
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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