XB-CLONE-3539219
Clone Name: IMAGE:7719772
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Gene:
coq6
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Species: Xenopus tropicalis
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Sequence:
5' EST: CX982931 [+]
cttatccagactcctgcggatgctgtctcagtctcaaggccgagctttgagctccacaggtcctgctgtatatgatgtggttatatctggtggtggtatg
gttggaactgcaatggcctgtgcattgggctcggatcctcacttgcagcacaagaaggtacttttgctagaagctgggaatagaaaaccatttgatcatc
tacctgagaacttcagcaacagagtgagctccattaccccaggctcagctaccctacttgccagctttggtgcttgggatcatatcttggccatgaggct
aaaaccatataagagaatgcaggtttgggatgcctgctcagatgcgctgatcacatttgataaggatgcattggaagatatgggatatattgtggaaaat
gatataattattgaagcacttacaaagcagctggaattaatgtctgatcatgttgaggttatgtacagaagcagagctttgagctactcatggcctccac
catataataatggcaaagccacaccttgggtagagatagagctagcagatggacaaaggcttcacacaaaactcttggtaaggtgttttctggtatgctg
catacctgtgttttacccccttcctaaaatattaagtattttcagattggtgcagatggccacaactccatggtgcgcagtgcagctggaatgcagagtg
tccagtggaattataatcatgctgctgtagttgctacacttcatttgtctgaggcaacagataacaatgttgcctggcagaggttccttcccactgggcc
catcgcattactgcctctttctgacacatgtagttctttagtgtggtctacatctcctgaacatgcatcagagcttgtcagcatggatgag
3' EST: CX982930 [+]aaatataagataataaattatattctatccctacttacaagccacagaataaaaaaaagtcaaagttaatttcagggttaactacccttttaacattatg
aatatgctaaggaaaaaaatagaggatcattcccacggcactgtggaatgggcctttaatgtgactttttgtacaaccgaagtctgagaagctacaggca
acgattaacaagcaaaataaaaacaaaaaaagatatttcagaggcatctgcagaaaataaatcaatatttatctttaatcaaaaatagtaaaccttgaat
tattttaatcagaaactgaatccaaattgatcttcttatttgcttgcaaaggccataatctgctcttttactggagtcagtgcatttgtagcttgcaggc
caagcgtcctgagaagtacaattggtggctgttttgtgttgtataatctctttagcagatcaactgcagccattaaaggaagattgtgcctctgtcgctc
agtctcatattctagaaggtgctttgtagatcccaggtcacttccattgaatgcagcttgactcagatgatgggccaaacaggcaacatctccaaagccc
atatttactccttgacctgccaaagggtgcactctgtgtgcggcgtcaccaattaatgccactcgatgccggatatattcagtggcatgtttcagtccca
gaggaaaggaagccctactgttctgctcaacacgtgacacactaggaggaagctgacgaggagaggagccagttggcatgaagaaagacaaagctgaatg
aagtagagaaccagctgaagttatgaactctgaatgattttcgtttgctcaaaatgctgaaatcactgtatctacaaagctttcctcatccatgctgac
Library:
Description: Library prepared from 5 ug of poly A+ RNA by oligo-dT priming [5'-GA(10)CTAGTCTCGAGT(18)-3'] and Stratascript reverse transcriptase. After ligation of EcoRI adapters (5'-AATTCGGCACGAGG-3') followed by kinasing adapters and XhoI digestion, the cDNA was size-selected by chromatography on Sepharose CL-2B columns and fractions containing cDNAs larger than 1 kb were ligated into EcoRI/XhoI digested pCS107 vector. Average insert size is 2.1 kb. The original library contained 9E4 recombinants. Reference: Current Genomics 4, 635-644. Use sp6 to obtain 5' end sequence, and t7 or t3 to obtain 3' end sequence. Library constructed by Bruce Blumberg (University of California, Irvine, Dept of Developmental and Cell Biology).Vector Details | Vector Map |
Name: pCS107
Type: plasmid
5' Restriction Site: EcoRI
3' Restriction Site: XhoI | |
Usage:
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